LOCUS       sequence-57180-e         961 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 961)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 961)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: IMPA2A
FEATURES             Location/Qualifiers
     source          1..961
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaagccgag cggcgaggac caggcggcgc tggcggccgg cccctgggag
      121 gagtgcttcc aggcggccgt gcagctggcg ctgcgggcag gacagatcat cagaaaagcc
      181 cttactgagg aaaaacgtgt ctcaacaaaa acatcagctg cagatcttgt gacagaaaca
      241 gatcaccttg tggaagattt aattatttct gagttgcgag agaggtttcc ttcacacagg
      301 ttcattgcag aagaggccgc ggcttctggg gccaagtgtg tgctcaccca cagcccgacg
      361 tggatcatcg accccatcga cggcacctgc aattttgtgc acagattccc gactgtggcg
      421 gttagcattg gatttgctgt tcgacaagag cttgaattcg gagtgattta ccactgcaca
      481 gaggagcggc tgtacacggg ccggcggggt cggggcgcct tctgcaatgg ccagcggctc
      541 cgggtctccg gggagacaga tctctcaaag gccttggttc tgacagaaat tggccccaaa
      601 cgtgaccctg cgaccctgaa gctgttcctg agtaacatgg agcggctgct gcatgccaag
      661 gcgcatgggg tccgagtgat tggaagctcc acattggcac tctgccacct ggcctcaggg
      721 gccgcggatg cctattacca gtttggcctg cactgctggg atctggcggc tgccacagtc
      781 atcatcagag aagcaggcgg catcgtgata gacacttcgg gtggacccct cgacctcatg
      841 gcttgcagag tggttgcggc cagcacccgg gagatggcga tgctcatagc tcaggcctta
      901 cagacgatta actatgggcg ggatgatgag aagtgacagt aaaggtggat acggatccga
      961 a
//
