LOCUS       sequence-57184-e         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX2AA
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggaaggcgt catcgagagt aactggaatg agattgttga cagctttgat
      121 gacatgaacc tctcggagtc ccttctccgt ggcatctacg cctatggttt tgagaagccc
      181 tctgccatcc agcagcgagc cattctacct tgtatcaagg gttatgatgt gattgctcaa
      241 gcccaatctg ggactgggaa aacggccaca tttgccatat caattctgca gcagattgaa
      301 ttagatctaa aagccaccca ggccttggtc ctagcaccca ctcgagaatt ggctcagcag
      361 atacagaagg tggtcatggc actaggagac tacatgggcg cctcctgtca cgcctgtatc
      421 gggggcacca acgtgcgtgc tgaggtgcag aaactgcaga tggaagctcc ccacatcatc
      481 gtgggtaccc ctggccgtgt gtttgatatg cttaaccgga gatacctgtc ccccaaatac
      541 atcaagatgt ttgtactgga tgaagctgac gaaatgttaa gccgtggatt caaggaccag
      601 atctatgaca tattccaaaa gctcaacagc aacacccagg tagttttgct gtcagccaca
      661 atgccttctg atgtgcttga ggtgaccaag aagttcatga gggaccccat tcggattctt
      721 gtcaagaagt gacagtaaag gtggatacgg atccgaa
//
