LOCUS       sequence-57185-e        1270 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1270)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIP5K2CA
FEATURES             Location/Qualifiers
     source          1..1270
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcatttcgt gcagcagaag gtgaaggtgt tccgggcggc cgacccgctg
      121 gtgggtgtgt tcctgtgggg cgtagcccac tcgatcaatg agctcagcca ggtgcctccc
      181 ccggtgatgc tgctgccaga tgactttaag gccagctcca agatcaaggt caacaatcac
      241 cttttccaca gggaaaatct gcccagtcat ttcaagttca aggagtattg tccccaggtc
      301 ttcaggaacc tccgtgatcg atttggcatt gatgaccaag attacttggt gtcccttacc
      361 cgaaaccccc ccagcgaaag tgaaggcagt gatggtcgct tccttatctc ctacgatcgg
      421 actctggtca tcaaagaagt atccagtgag gacattgctg acatgcatag caacctctcc
      481 aactatcacc agtacattgt gaagtgccat ggcaacacgc ttctgcccca gttcctgggg
      541 atgtaccgag tcagtgtgga caacgaagac agctacatgc ttgtgatgcg caatatgttt
      601 agccaccgtc ttcctgtgca caggaagtat gacctcaagg gttccctagt gtcccgggaa
      661 gccagcgata aggaaaaggt taaagaattg cccaccctta gggatatgga ctttctcaac
      721 aagaaccaga aagtatatat tggtgaagag gagaagaaaa tatttctgga gaagctgaag
      781 agagatgtgg agtttctagt gcagctgaag atcatggact acagccttct gctaggcatc
      841 cacgacatca ttcggggctc tgaaccagag gaggaagcgc ccgtgcggga ggatgagtca
      901 gaggtggatg gggactgcag cctgactgga cctcctgctc tggtgggctc ctatggcacc
      961 tccccagagg gtatcggagg ctacatccat tcccatcggc ccctgggccc aggagagttt
     1021 gagtccttca ttgatgtcta tgccatccgg agtgctgaag gagcccccca gaaggaggtc
     1081 tacttcatgg gcctcattga tatccttaca cagtatgatg ccaagaagaa agcagctcat
     1141 gcagccaaaa ctgtcaagca tggggctggg gcagagatct ctactgtcca tccggagcag
     1201 tatgctaagc gattcctgga ttttattacc aacatctttg cctaacagta aaggtggata
     1261 cggatccgaa
//
