LOCUS       sequence-57188-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NT5C3A
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             13..30
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             40..57
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 atgggcagca gccatcatca tcatcatcac agcagcggcc tggttccgcg tggtagtaac
       61 cctacaagag tagaagaaat tatctgtggt cttatcaaag gaggagctgc caaacttcag
      121 ataataacgg actttgatat gacactcagt agattttcat ataaagggaa aagatgccca
      181 acatgtcata atatcattga caactgtaag ctggttacag atgaatgtag aaaaaagtta
      241 ttgcaactaa aggaaaaata ctacgctatt gaagttgatc ctgttcttac tgtagaagag
      301 aagtaccctt atatggtgga atggtatact aaatcacatg gtttgcttgt tcagcaagct
      361 ttaccaaaag ctaaacttaa agaaattgtg gcagaatctg acgttatgct caaagaagga
      421 tatgagaatt tctttgataa gctccaacaa catagcatcc ccgtgttcat attttcggct
      481 ggaatcggcg atgtactaga ggaagttatt cgtcaagctg gtgtttatca tcccaatgtc
      541 aaagttgtgt ccaattttat ggattttgat gaaactgggg tgctcaaagg atttaaagga
      601 gaactaattc atgtatttaa caaacatgat ggtgccttga ggaatacaga atatttcaat
      661 caactaaaag acaatagtaa cataattctt ctgggagact cccaaggaga cttaagaatg
      721 gcagatggag tggccaatgt tgagcacatt ctgaaaattg gatatctaaa tgatagagtg
      781 gatgagcttt tagaaaagta catggactct tatgatattg ttttagtaca agatgaatca
      841 ttagaagtag ccaactctat tttacagaag attctatgat gacgaagctt g
//
