LOCUS       sequence-57189-e         466 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 466)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 466)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: THEM2A
FEATURES             Location/Qualifiers
     source          1..466
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcgcaattt tgagagagtt ttgggaaaga ttactcttgt ctctgctgct
      121 cctgggaaag tgatttgtga aatgaaagta gaagaagagc ataccaatgc aataggcact
      181 ctccacggcg gtttgacagc cacgttagta gataacatat caacaatggc tctgctatgc
      241 acggaaaggg gagcacccgg agtcagtgtc gatatgaaca taacgtacat gtcacctgca
      301 aaattaggag aagatatagt gattacagca catgttctga agcaaggaaa aacacttgca
      361 tttacctctg tggatctgac caacaaggcc acaggaaaat taatagcaca aggaagacac
      421 acaaaacacc tgggaaactg acagtaaagg tggatacgga tccgaa
//
