LOCUS       sequence-57190-e         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EPB41L3A
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaagcctaa aagcatgcag tgcaaagtga tacttctcga tggatcagaa
      121 tatacctgtg atgtagagaa acgctccaga ggacaagtgc tgtttgataa agtgtgtgaa
      181 cacttgaact tgctagagaa agactacttt gggcttacgt atcgagatgc tgaaaaccag
      241 aagaattggt tggaccctgc taaggaaata aaaaaacagg ttcgaagtgg tgcttggcac
      301 ttttcattta atgtgaaatt ttatccacca gaccctgccc aactatctga agatatcacc
      361 aggtactacc tctgcttgca gttgcgagat gacatcgtgt ccggaaggct gccctgctcc
      421 tttgttaccc tggccttgct gggctcctac actgtccagt cagagctcgg agactatgac
      481 ccagatgaat gtgggagcga ttacattagt gagttccgct ttgcaccaaa ccacactaaa
      541 gaactggaag acaaagtgat cgagctgcac aagagccaca gaggaatgac gccagcagaa
      601 gcagagatgc atttcttgga aaatgccaaa aaattatcaa tgtatggggt agatttacat
      661 catgctaagg actcagaagg ggtagaaatt atgttaggag tttgtgcaag tggtctgttg
      721 atatatcgcg accggctgcg aataaacaga tttgcctggc ccaaggttct aaagatttca
      781 tacaaacgga acaactttta cattaagatc cggccgggag agtttgaaca atttgaaagc
      841 accattgggt ttaagctgcc aaaccatcga gctgccaagc gtttatggaa agtatgtgtt
      901 gagcatcata catttttcag actactgtta ccagaagcac ctcccaagtg acagtaaagg
      961 tggatacgga tccgaa
//
