LOCUS       sequence-57191-e        1414 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1414)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SCLYA
FEATURES             Location/Qualifiers
     source          1..1414
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggggaggga tgcgccggca cccgcggcga gtcagcccag cggctgcggg
      121 aaacacaact cgccggagag gaaagtttat atggactata atgcaacgac tcccctggag
      181 ccagaagtta tccaggccat gaccaaggcc atgtgggaag cctggggaaa tcccagcagc
      241 ccgtattcag caggaagaaa ggccaaggat attataaatg cagctcggga aagcctcgcg
      301 aagatgatag gggggaaacc tcaagatata atcttcactt ccgggggcac tgagtcaaat
      361 aatttagtaa tccattctgt ggtgaaacat ttccacgcaa accagacctc aaagggacac
      421 acaggtgggc accacagccc agtgaagggg gccaagcccc atttcattac ttcctcggtg
      481 gaacacgact ccatccggct gcccctggag cacctggtgg aagaacaagt ggcagcggtc
      541 acctttgtcc cggtgtccaa ggtgagcggg cagacagagg tggacgacat cctcgcggca
      601 gtccgcccga ccacacgcct cgtgaccatc atgctggcca acaatgagac tggcattgtc
      661 atgcctgtcc ctgaaatcag tcagcgcatt aaagccctga accaggaacg ggtggcagct
      721 gggctacctc ccatcctcgt gcacacggat gctgcacagg ccttggggaa gcagcgcgtg
      781 gatgtggagg acctgggcgt ggacttcctt acaatcgtgg ggcacaagtt ttatggtccc
      841 aggattggcg cactttatat acgaggactt ggtgaattta cccctctcta ccctatgcta
      901 tttggaggtg gacaagaacg gaatttcagg ccagggacag agaacacccc aatgattgct
      961 ggccttggga aggccgcgga gctggtgacc cagaactgcg aggcttatga ggcccacatg
     1021 agggacgtcc gcgactacct ggaagagagg ctggaagctg aattcggtca gaagagaatc
     1081 catctgaata gccagtttcc aggcacccag cggcttccca atacctgtaa cttttccatc
     1141 cggggacccc ggcttcaagg ccacgtggtg cttgcgcagt gccgagtgct gatggccagt
     1201 gtgggggccg cgtgccactc ggaccacggg gaccagccgt ccccagtgct gctgagctac
     1261 ggtgtcccct tcgacgtggc caggaacgcg ctccggctca gcgtgggccg cagcaccacc
     1321 agggccgagg tggacctcgt cgtgcaggac ctgaagcagg ccgtggcgca gctggaggac
     1381 caggcctgac agtaaaggtg gatacggatc cgaa
//
