LOCUS       sequence-57193-e         463 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 463)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 463)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PHPT1A
FEATURES             Location/Qualifiers
     source          1..463
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggacctcgc tctcattcct gatgtggaca tcgactccga cggcgtcttc
      121 aagtatgtgc tgatccgagt ccactcggct ccccgctccg gggctccggc tgcagagagc
      181 aaggagatcg tgcgcggcta caagtgggct gagtaccatg cggacatcta cgacaaagtg
      241 tcgggcgaca tgcagaagca aggctgcgac tgtgagtgtc tgggcggcgg gcgcatctcc
      301 caccagagtc aggacaagaa gattcacgtg tacggctatt ccatggccta tggtcctgcc
      361 cagcacgcca tttcaactga gaaaatcaaa gccaagtacc ccgactacga ggtcacctgg
      421 gctaacgacg gctactgaca gtaaaggtgg atacggatcc gaa
//
