LOCUS       sequence-57195-e        1345 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1345)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1345)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX3XA
FEATURES             Location/Qualifiers
     source          1..1345
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggttgaggc aacaggcaac aactgtcctc cacatattga aagtttcagt
      121 gatgttgaga tgggagaaat tatcatggga aacattgagc ttactcgtta tactcgccca
      181 actccagtgc aaaagcatgc tattcctatt atcaaagaga aaagagactt gatggcttgt
      241 gcccaaacag ggtctggaaa aactgcagca tttctgttgc ccatcttgag tcagatttat
      301 tcagatggtc caggcgaggc tttgagggcc atgaaggaaa atggaaggta tgggcgccgc
      361 aaacaatacc caatctcctt ggtattagca ccaacgagag agttggcagt acagatctac
      421 gaggaagcca gaaaattttc ataccgatct agagttcgtc cttgcgtggt ttatggtggt
      481 gccgatattg gtcagcagat tcgagacttg gaacgtggat gccatttgtt agtagccact
      541 ccaggacgtc tagtggatat gatggaaaga ggaaagattg gattagactt ttgcaaatac
      601 ttggtgttag atgaagctga tcggatgttg gatatggggt ttgagcctca gattcgtaga
      661 atagtcgaac aagatactat gcctccaaag ggtgtccgcc acactatgat gtttagtgct
      721 acttttccta aggaaataca gatgctggct cgtgatttct tagatgaata tatcttcttg
      781 gctgtaggaa gagttggctc tacctctgaa aacatcacac agaaagtagt ttgggtggaa
      841 gaatcagaca aacggtcatt tctgcttgac ctcctaaatg caacaggcaa ggattcactg
      901 accttagtgt ttgtggagac caaaaagggt gcagattctc tggaggattt cttataccat
      961 gaaggatacg catgtaccag catccatgga gaccgttctc agagggatag agaagaggcc
     1021 cttcaccagt tccgctcagg aaaaagccca attttagtgg ctacagcagt agcagcaaga
     1081 ggactggaca tttcaaatgt gaaacatgtt atcaattttg acttgccaag tgatattgaa
     1141 gaatatgtac atcgtattgg tcgtacggga cgtgtaggaa accttggcct ggcaacctca
     1201 ttctttaacg agaggaacat aaatattact aaggatttgt tggatcttct tgttgaagct
     1261 aaacaagaag tgccgtcttg gttagaaaac atggcttatg aacaccacta caagggttga
     1321 cagtaaaggt ggatacggat ccgaa
//
