LOCUS       sequence-57198-e        1303 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1303)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CTHA
FEATURES             Location/Qualifiers
     source          1..1303
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcaggaaaa agacgcctcc tcacaaggtt tcctgccaca cttccaacat
      121 ttcgccacgc aggcgatcca tgtgggccag gatccagagc aatggacctc cagggctgta
      181 gtgcccccca tctcactgtc caccacgttc aagcaagggg cgcctggcca gcactcgggt
      241 tttgaatata gccgttctgg aaatcccact aggaattgcc ttgaaaaagc agtggcagca
      301 ctggatgggg ctaagtactg tttggccttt gcttcaggtt tagcagccac tgtaactatt
      361 acccatcttt taaaagcagg agaccaaatt atttgtatgg atgatgtgta tggaggtaca
      421 aacaggtact tcaggcaagt ggcatctgaa tttggattaa agatttcttt tgttgattgt
      481 tccaaaatca aattactaga ggcagcaatt acaccagaaa ccaagcttgt ttggatcgaa
      541 acccccacaa accccaccca gaaggtgatt gacattgaag gctgtgcaca tattgtccat
      601 aagcatggag acattatttt ggtcgtggat aacactttta tgtcaccata tttccagcgc
      661 cctttggctc tgggagctga tatttctatg tattctgcaa caaaatacat gaatggccac
      721 agtgatgttg taatgggcct ggtgtctgtt aattgtgaaa gccttcataa tagacttcgt
      781 ttcttgcaaa actctcttgg agcagttcca tctcctattg attgttacct ctgcaatcga
      841 ggtctgaaga ctctacatgt ccgaatggaa aagcatttca aaaacggaat ggcagttgcc
      901 cagttcctgg aatctaatcc ttgggtagaa aaggttattt atcctgggct gccctctcat
      961 ccacagcatg agttggtgaa gcgtcagtgt acaggttgta cagggatggt caccttttat
     1021 attaagggca ctcttcagca tgctgagatt ttcctcaaga acctaaagct atttactctg
     1081 gccgagagct tgggaggatt cgaaagcctt gctgagcttc cggcaatcat gactcatgca
     1141 tcagttctta agaatgacag agatgtcctt ggaattagtg acacactgat tcgactttct
     1201 gtgggcttag aggatgagga agacctactg gaagatctag atcaagcttt gaaggcagca
     1261 caccctccaa gtggatgaca gtaaaggtgg atacggatcc gaa
//
