LOCUS       sequence-57201-e        1333 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1333)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1333)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ASSA
FEATURES             Location/Qualifiers
     source          1..1333
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgtccagcaa aggctccgtg gttctggcct acagtggcgg cctggacacc
      121 tcgtgcatcc tcgtgtggct gaaggaacaa ggctatgacg tcattgccta tctggccaac
      181 attggccaga aggaagactt cgaggaagcc aggaagaagg cactgaagct tggggccaaa
      241 aaggtgttca ttgaggatgt cagcagggag tttgtggagg agttcatctg gccggccatc
      301 cagtccagcg cactgtatga ggaccgctac ctcctgggca cctctcttgc caggccctgc
      361 atcgcccgca aacaagtgga aatcgcccag cgggaggggg ccaagtatgt gtcccacggc
      421 gccacaggaa aggggaacga tcaggtccgg tttgagctca gctgctactc actggccccc
      481 cagataaagg tcattgctcc ctggaggatg cctgaattct acaaccggtt caagggccgc
      541 aatgacctga tggagtacgc aaagcaacac gggattccca tcccggtcac tcccaagaac
      601 ccgtggagca tggatgagaa cctcatgcac atcagctacg aggctggaat cctggagaac
      661 cccaagaacc aagcgcctcc aggtctctac acgaagaccc aggacccagc caaagccccc
      721 aacacccctg acattctcga gatcgagttc aaaaaagggg tccctgtgaa ggtgaccaac
      781 gtcaaggatg gcaccaccca ccagacctcc ttggagctct tcatgtacct gaacgaagtc
      841 gcgggcaagc atggcgtggg ccgtattgac atcgtggaga accgcttcat tggaatgaag
      901 tcccgaggta tctacgagac cccagcaggc accatccttt accacgctca tttagacatc
      961 gaggccttca ccatggaccg ggaagtgcgc aaaatcaaac aaggcctggg cttgaaattt
     1021 gctgagctgg tgtataccgg tttctggcac agccctgagt gtgaatttgt ccgccactgc
     1081 atcgccaagt cccaggagcg agtggaaggg aaagtgcagg tgtccgtcct caagggccag
     1141 gtgtacatcc tcggccggga gtccccactg tctctctaca atgaggagct ggtgagcatg
     1201 aacgtgcagg gtgattatga gccaactgat gccaccgggt tcatcaacat caattccctc
     1261 aggctgaagg aatatcatcg tctccagagc aaggtcactg ccaaatgaca gtaaaggtgg
     1321 atacggatcc gaa
//
