LOCUS       sequence-57210-e        1168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1168)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PRPSAP2A
FEATURES             Location/Qualifiers
     source          1..1168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             647..658
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaacataac caaaggtggt ctggtgttgt tttcagcaaa ctcgaattca
      121 tcatgtatgg agctatcaaa gaaaattgca gagcggctag gggtggagat gggcaaagtg
      181 caggtttacc aggaacctaa cagagaaaca agagtacaaa ttcaagagtc tgtgagggga
      241 aaagatgttt tcatcatcca aactgtttcg aaggacgtga acaccaccat catggagctc
      301 ctgatcatgg tgtatgcatg taagacctct tgtgccaaga gcatcattgg cgtgataccc
      361 tactttcctt acagcaagca gtgcaagatg agaaaaagag gctccattgt ctctaaattg
      421 ctggcttcca tgatgtgcaa agctggtcta actcatctta ttactatgga tttacaccag
      481 aaggaaattc agggcttctt caatattcct gttgacaatt taagagcatc tcccttctta
      541 ttacagtata ttcaagaaga gatcccagat tacaggaatg cagtaatcgt ggccaagtct
      601 ccagcctcgg cgaagagggc acagtctttt gctgagcgcc tgcgcctggg aattgcagtg
      661 attcatggag aggcgcagga tgccgagtcg gacttggtgg atggacggca ttccccaccc
      721 atggtcagaa gtgtggctgc catccacccc agcctggaga tccccatgct gattcctaaa
      781 gaaaagcccc caatcacggt tgtgggtgat gttggaggaa ggattgccat catcgtggat
      841 gacatcattg atgatgttga cagctttctt gctgcagcag agaccctgaa ggaaagaggt
      901 gcatataaga tctttgtgat ggcaactcat ggcttgttgt cttctgacgc cccccggcgg
      961 attgaagagt ctgccattga tgaggtggtg gtcaccaata caattccaca tgaagtccag
     1021 aagctccagt gccccaagat taaaactgtg gatatcagca tgatcctttc agaggcgatc
     1081 cgtcggatcc acaatgggga gtccatgtcc taccttttca gaaacatagg cttagatgac
     1141 tgacagtaaa ggtggatacg gatccgaa
//
