LOCUS       sequence-57215-e        1459 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1459)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1459)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GUADA
FEATURES             Location/Qualifiers
     source          1..1459
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgtgtgccgc tcagatgccg cccctggcgc acatcttccg agggacgttc
      121 gtccactcca cctggacctg ccccatggag gtgctgcggg atcacctcct cggcgtgagc
      181 gacagcggca aaatagtgtt tttagaagaa gcatctcaac aggaaaaact ggccaaagaa
      241 tggtgcttca agccgtgtga aataagagaa ctgagccacc atgagttctt catgcctggg
      301 ctggttgata cacacatcca tgcctctcag tattcctttg ctggaagtag catagacctg
      361 ccactcttgg agtggctgac caagtacaca tttcctgcag aacacagatt ccagaacatc
      421 gactttgcag aagaagtata taccagagtt gtcaggagaa cactaaagaa tggaacaacc
      481 acagcttgtt actttgcaac aattcacact gactcatctc tgctccttgc cgacattaca
      541 gataaatttg gacagcgggc atttgtgggc aaagtttgca tggatttgaa tgacactttt
      601 ccagaataca aggagaccac tgaggaatcg atcaaggaaa ctgagagatt tgtgtcagaa
      661 atgctccaaa agaactattc tagagtgaag cccatagtga caccacgttt ttccctctcc
      721 tgctctgaga ctttgatggg tgaactgggc aacattgcta aaacccgtga tttgcacatt
      781 cagagccata taagtgaaaa tcgtgatgaa gttgaagctg tgaaaaactt ataccccagt
      841 tataaaaact acacatctgt gtatgataaa aacaatcttt tgacaaataa gacagtgatg
      901 gcacacggct gctacctctc tgcagaagaa ctgaacgtat tccatgaacg aggagcatcc
      961 atcgcacact gtcccaattc taatttatcg ctcagcagtg gatttctaaa tgtgctagaa
     1021 gtcctgaaac atgaagtcaa gatagggctg ggtacagacg tggctggtgg ctattcatat
     1081 tccatgcttg atgcaatcag aagagcagtg atggtttcca atatcctttt aattaataag
     1141 gtaaatgaga aaagcctcac cctcaaagaa gtcttcagac tagctactct tggaggaagc
     1201 caagccctgg ggctggatgg tgagattgga aactttgaag tgggcaagga atttgatgcc
     1261 atcctgatca accccaaagc atccgactct cccattgacc tgttttatgg ggactttttt
     1321 ggtgatattt ctgaggctgt tatccagaag ttcctctatc taggagatga tcgaaatatt
     1381 gaagaggttt atgtgggcgg aaagcaggtg gttccgtttt ccagctcagt gtaacagtaa
     1441 aggtggatac ggatccgaa
//
