LOCUS       sequence-57216-e         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP12A
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggactcctc tgccctgcca gacccaggct ttcagaagat cacccttagt
      121 tcttcctcgg aagagtatca gaaggtctgg aacctcttta accgcacgct gcctttctac
      181 tttgttcaga agattgagcg agtacagaac ctggccctct gggaagtcta ccagtggcaa
      241 aaaggacaga tgcagaagca gaatggaggg aaggccgtgg acgagcggca gctgttccac
      301 ggcaccagcg ccatttttgt ggacgccatc tgccagcaga actttgactg gcgggtctgt
      361 ggtgttcatg gcacttccta cggcaagggg agctactttg cccgagatgc tgcatattcc
      421 caccactaca gcaaatccga cacgcagacc cacacgatgt tcctggcccg ggtgctggtg
      481 ggcgagttcg tcaggggcaa tgcgtccttt gtccgtccgc cggccaagga gggctggagc
      541 aacgccttct atgatagctg cgtgaacagt gtgtccgacc cctccatctt tgtgatcttt
      601 gagaaacacc aggtctaccc agagtatgtc atccagtaca ccacctcctc caagccctcg
      661 tgacagtaaa ggtggatacg gatccgaa
//
