LOCUS       sequence-57219-e        1408 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1408)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ADSS2A
FEATURES             Location/Qualifiers
     source          1..1408
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcccagggc gcggcccgga ggaaaccggg tgacggtggt gctcggtgcg
      121 cagtggggcg acgaaggcaa agggaaggtg gtggacctgc tggcgcagga cgccgacatc
      181 gtgtgccgct gccagggagg aaataatgct ggccatacag ttgttgtgga ttctgtggaa
      241 tatgattttc atctcttacc cagtggaata attaatccaa atgtcactgc attcattgga
      301 aatggtgtgg taattcatct acctggattg tttgaagaag cagagaaaaa tgttcaaaaa
      361 ggaaaaggac tagaaggctg ggaaaaaagg cttattatat ctgacagagc tcatattgta
      421 tttgattttc atcaagcagc tgatggtatc caggaacaac agagacaaga acaagcagga
      481 aaaaatttgg gtacaacaaa aaagggcatt ggcccagttt attcgtccaa agctgctcgg
      541 agtggactca ggatgtgcga ccttgtttct gactttgatg gcttctctga gaggtttaaa
      601 gttctagcta accaatacaa atctatatac cccactttgg aaatagacat tgaaggtgaa
      661 ttacaaaaac tcaagggtta tatggaaaag attaaaccaa tggtgagaga tggagtttat
      721 tttctatatg aggccctaca tggaccacca aagaaaatct tggtagaagg tgcaaatgca
      781 gcactattag atattgattt tgggacttac ccttttgtaa cctcttcaaa ttgtactgtt
      841 ggaggtgttt gtactggttt gggtatgcca cctcaaaatg ttggagaagt gtatggagtt
      901 gtgaaagctt atacaactag agttggtatt ggtgcctttc ctacagagca agacaatgaa
      961 attggagaat tattacaaac aaggggtaga gagtttggtg taactactgg aaggaaaaga
     1021 agatgtggct ggttggacct cgttttgctc aaatatgctc atatgatcaa tggatttact
     1081 gcgttggcac ttaccaagtt ggatattttg gacatgttta cggaaatcaa agttggagtt
     1141 gcttacaagt tagatggtga aatcatacct catatcccag caaaccaaga agtcttaaat
     1201 aaagttgaag ttcaatataa gactctccca ggatggaaca cagacatatc aaatgcaagg
     1261 gcgtttaaag aactacctgt taatgcacaa aactatgttc gatttattga agatgagctt
     1321 caaattccag ttaagtggat tggtgttggt aaatccagag aatctatgat tcaactcttt
     1381 tgacagtaaa ggtggatacg gatccgaa
//
