LOCUS       sequence-57220-e        1576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1576)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CSADA
FEATURES             Location/Qualifiers
     source          1..1576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggctgactc agaagcactc ccctcccttg ctggggaccc agtggctgtg
      121 gaagccttgc tccgggccgt gtttggggtt gttgtggatg aggccattca gaaaggaacc
      181 agtgtctccc agaaggtctg tgagtggaag gagcctgagg agctgaagca gctgctggat
      241 ttggagctgc ggagccaggg cgagtcacag aagcagatcc tggagcggtg tcgggctgtg
      301 attcgctaca gtgtcaagac tggtcaccct cggttcttca accagctctt ctctgggttg
      361 gatccccatg ctctggccgg gcgcattatc actgagagcc tcaacaccag ccagtacaca
      421 tatgaaatcg cccccgtgtt tgtgctcatg gaagaggagg tgctgaggaa actgcgggcc
      481 ctggtgggct ggagctctgg ggacggaatc ttctgccctg gtggctccat ctccaacatg
      541 tatgctgtaa atctggcccg ctatcagcgc tacccggatt gcaagcagag gggcctccgc
      601 acactgccgc ccctggccct attcacatcg aaggagtgtc actactccat ccagaaggga
      661 gctgcgtttc tgggacttgg caccgacagt gtccgagtgg tcaaggctga tgagagaggg
      721 aaaatggtcc ccgaggatct ggagaggcag attggtatgg ccgaggctga gggtgctgtg
      781 ccgttcctgg tcagtgccac ctctggcacc actgtgctag gggcctttga ccccctggag
      841 gcaattgctg atgtgtgcca gcgtcatggg ctatggctgc atgtggatgc tgcctggggt
      901 gggagcgtcc tgctgtcaca gacacacagg catctcctgg atgggatcca gagggctgac
      961 tctgtggcct ggaatcccca caagctcctc gcagcaggcc tgcaatgctc tgcacttctt
     1021 ctccaggata cctcgaacct gctcaagcgc tgccatgggt cccaggccag ctaccttttc
     1081 cagcaggaca agttctacga tgtggctctg gacacgggag acaaggtggt gcagtgtggc
     1141 cgccgtgtgg actgtctgaa gctgtggctc atgtggaagg cacagggcga tcaagggctg
     1201 gagcggcgca tcgaccaggc ctttgtcctt gcccggtacc tggtggagga aatgaagaag
     1261 cgggaagggt ttgagctagt catggagcct gagtttgtca atgtgtgttt ctggttcgta
     1321 ccccccagcc tgcgagggaa gcaggagagt ccagattacc acgaaaggct gtcaaaggtg
     1381 gcccccgtgc tcaaggagcg catggtgaag gagggctcca tgatgattgg ctaccagccc
     1441 cacgggaccc ggggcaactt cttccgtgtg gttgtggcca actctgcact gacctgtgct
     1501 gatatggact tcctcctcaa cgagctggag cggctaggcc aggacctgtg acagtaaagg
     1561 tggatacgga tccgaa
//
