LOCUS       sequence-57221-e        1387 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1387)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1387)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GARTA
FEATURES             Location/Qualifiers
     source          1..1387
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggcagcccg agtacttata attggcagtg gaggaaggga acatacgctg
      121 gcctggaaac ttgcacagtc tcatcatgtc aaacaagtgt tggttgcccc aggaaacgca
      181 ggcactgcct gctctgaaaa gatttcaaat accgccatct caatcagtga ccacactgcc
      241 cttgctcaat tctgcaaaga gaagaaaatt gaatttgtag ttgttggacc agaagcacct
      301 ctggctgctg ggattgttgg gaacctgagg tctgcaggag tgcaatgctt tggcccaaca
      361 gcagaagcgg ctcagttaga gtccagcaaa aggtttgcca aagagtttat ggacagacat
      421 ggaatcccaa ccgcacaatg gaaggctttc accaaacctg aagaagcctg cagcttcatt
      481 ttgagtgcag acttccctgc tttggttgtg aaggccagtg gtcttgcagc tggaaaaggg
      541 gtgattgttg caaagagcaa agaagaggcc tgcaaagctg tacaagagat catgcaggag
      601 aaagcctttg gggcagctgg agaaacaatt gtcattgaag aacttcttga cggagaagag
      661 gtgtcgtgtc tgtgtttcac tgatggcaag actgtggccc ccatgccccc agcacaggac
      721 cataagcgat tactggaggg agatggtggc cctaacacag ggggaatggg agcctattgt
      781 ccagcccctc aggtttctaa tgatctatta ctaaaaatta aagatactgt tcttcagagg
      841 acagtggatg gcatgcagca agagggtact ccatatacag gtattctcta tgctggaata
      901 atgctgacca agaatggccc aaaagttcta gagtttaatt gccgttttgg tgatccagag
      961 tgccaagtaa tcctcccact tcttaaaagt gatctttatg aagtgattca gtccacctta
     1021 gatggactgc tctgcacatc tctgcctgtt tggctagaaa accacaccgc cctaactgtt
     1081 gtcatggcaa gtaaaggtta tcctggagac tacaccaagg gtgtagagat aacagggttt
     1141 cctgaggctc aagctctagg actggaggtg ttccatgcag gcactgccct caaaaatggc
     1201 aaagtagtaa ctcatggggg tagagttctt gcagtcacag ccatccggga aaatctcata
     1261 tcagcccttg aggaagccaa gaaaggacta gctgctataa agtttgaggg agcaatttat
     1321 aggaaagaca tcggctttcg tgccatagct ttcctccagt gacagtaaag gtggatacgg
     1381 atccgaa
//
