LOCUS       sequence-57222-e         610 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 610)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT7A
FEATURES             Location/Qualifiers
     source          1..610
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgggggacat cgtccagcca gtcctcaacc cagagccgaa cactgtcagc
      121 tacagccagt ccagcttgat ccacctggtg gggccttcag actgcaccct gcacggcttt
      181 gtgcacggag gtgtgaccat gaagctcatg gatgaggtcg ccgggatcgt ggctgcacgc
      241 cactgcaaga ccaacatcgt cacagcttcc gtggacgcca ttaattttca tgacaagatc
      301 agaaaaggct gcgtcatcac catctcggga cgcatgacct tcacgagcaa taagtccatg
      361 gagatcgagg tgttggtgga cgccgaccct gttgtggaca gctctcagaa gcgctaccgg
      421 gccgccagtg ccttcttcac ctacgtgtcg ctgagccagg aaggcaggtc gctgcctgtg
      481 ccccagctgg tgcccgagac cgaggacgag aagaagcgct ttgaggaagg caaagggcgg
      541 tacctgcaga tgaaggcgaa gcgacagggc cacgcggagc cttgacagta aaggtggata
      601 cggatccgaa
//
