LOCUS       sequence-57227-e         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MDA5A
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaacagcaa catgggcagt gattcaggca ccatgggaag tgattcagat
      121 gaagagaatg tggcagcaag agcatccccg gagccagaac tccagctcag gccttaccaa
      181 atggaagttg cccagccagc cttggaaggg aagaatatca tcatctgcct ccctacaggg
      241 agtggaaaaa ccagagtggc tgtttacatt gccaaggatc acttagacaa gaagaaaaaa
      301 gcatctgagc ctggaaaagt tatagttctt gtcaataagg tactgctagt tgaacagctc
      361 ttccgcaagg agttccaacc atttttgaag aaatggtatc gtgttattgg attaagtggt
      421 gatacccaac tgaaaatatc atttccagaa gttgtcaagt cctgtgatat tattatcagt
      481 acagctcaaa tccttgaaaa ctccctctta aacttggaaa atggagaaga tgctggtgtt
      541 caattgtcag acttttccct cattatcatt gatgaatgtc atcacaccaa caaagaagca
      601 gtgtataata acatcatgag gcattatttg atgcagaagt tgaaaaacaa tagactcaag
      661 aaagaaaaca aaccagtgat tccccttcct cagatactgg gactaacagc ttcatgacag
      721 taaaggtgga tacggatccg aa
//
