LOCUS       sequence-57231-e         694 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 694)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 694)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP15A
FEATURES             Location/Qualifiers
     source          1..694
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaatcttcc tgaacactgg actgacatga atcatcagct gttttgcatg
      121 gtccagctag agccaggaca atcagaatat aataccataa aggacaagtt cacccgaact
      181 tgttcttcct acgcaataga gaagattgag aggatacaga atgcatttct ctggcagagc
      241 taccaggtaa agaaaaggca aatggatatc aagaatgacc ataagaataa tgagagactc
      301 ctcttccatg ggacagatgc agactcagtg ccatatgtca atcagcacgg ctttaataga
      361 agttgtgctg ggaaaaatgc tgtatcctat ggaaaaggaa cctattttgc tgtggatgcc
      421 agttattctg ccaaggacac ctactccaag ccagacagca atgggagaaa gcacatgtac
      481 gttgtgcgag tacttactgg agtcttcaca aagggacgtg caggattagt cacccctcca
      541 cccaagaatc ctcacaatcc cacagatctc tttgactcag tgacaaacaa tacacgatct
      601 ccaaagctat ttgtggtatt ctttgataat caggcttacc cagaatatct cataactttc
      661 acggcttgac agtaaaggtg gatacggatc cgaa
//
