LOCUS       sequence-57235-e         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GMPSA
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggaaggagc tgttgtcatt ctggatgctg gtgctcagta cgggaaagtc
      121 atagaccgaa gagtgaggga actgttcgtg cagtctgaaa ttttcccctt ggaaacacca
      181 gcatttgcta taaaggaaca aggattccgt gctattatca tctctggagg acctaattct
      241 gtgtatgctg aagatgctcc ctggtttgat ccagcaatat tcactattgg caagcctgtt
      301 cttggaattt gctatggtat gcagatgatg aataaggtat ttggaggtac tgtgcacaaa
      361 aaaagtgtca gagaagatgg agttttcaac attagtgtgg ataatacatg ttcattattc
      421 aggggccttc agaaggaaga agttgttttg cttacacatg gagatagtgt agacaaagta
      481 gctgatggat tcaaggttgt ggcacgttct ggaaacatag tagcaggcat agcaaatgaa
      541 tctaaaaagt tatatggagc acagttccac cctgaagttg gccttacaga aaatggaaaa
      601 gtaatactga agaatttcct ttatgatata gctggatgca gtggaacctt caccgtgtga
      661 cagtaaaggt ggatacggat ccgaa
//
