LOCUS       sequence-57238-e        1039 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1039)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GLSA
FEATURES             Location/Qualifiers
     source          1..1039
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgattcctga ctttatgtct tttacatcgc acatcgacga gctgtacgag
      121 agtgctaaaa aacaatctgg cgggaaagtt gcggattata ttccgcagct ggccaaattt
      181 agtccggacc tgtggggagt ctcagtgtgt acggtagatg ggcagcgcca tagtaccggt
      241 gatactaaag ttccgttctg cctgcaaagc tgtgtgaaac cgctgaaata cgcgatcgcc
      301 gtcaacgacc tggggacaga gtatgttcac cgctacgtag gcaaagaacc gtcggggctg
      361 cgctttaata aactgtttct gaacgaagat gacaaaccgc ataatccgat ggtgaacgcg
      421 ggcgccattg tcgttacctc tctgatcaaa cagggggtga ataacgcgga gaaattcgat
      481 tatgtaatgc aatttctgaa taaaatggct ggaaacgaat acgtcggctt cagtaatgcc
      541 acgtttcagt cagagcgcga aagcggtgat cgcaacttcg caattgggta ttatctgaaa
      601 gaaaaaaaat gctttcctga gggcaccgac atggttggta tcctggattt ctactttcag
      661 ctgtgttcca ttgaagtgac ttgcgaatca gcgtctgtaa tggccgcaac actggcgaat
      721 ggaggcttct gtccgatcac gggtgagcgt gttctgagtc cagaagctgt gcgcaacacc
      781 ctgagcctga tgcactcatg cgggatgtat gacttttccg gccaatttgc cttccatgtc
      841 ggtctgccgg cgaaatcggg agttgctggc ggtattctgc tggtagtacc taatgtcatg
      901 gggatgatgt gttggtcgcc gccactggat aaaatgggga atagtgttaa aggtattcac
      961 ttttgccatg acctggtgag tctgtgtaat ttccataact atgataattt gtgacagtaa
     1021 aggtggatac ggatccgaa
//
