LOCUS       sequence-57242-e         598 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 598)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DEADC1A
FEATURES             Location/Qualifiers
     source          1..598
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggaggagac cgaaaagtgg atggaggagg cgatgcacat ggccaaagaa
      121 gccctcgaaa atactgaagt tcctgttggc tgtcttatgg tctacaacaa tgaagttgta
      181 gggaagggga gaaatgaagt taaccaaacc aaaaatgcta ctcgacatgc agaaatggtg
      241 gccatcgatc aggtcctcga ttggtgtcgt caaagtggca agagtccctc tgaagtattt
      301 gaacacactg tgttgtatgt cactgtggag ccgtgcatta tgtgtgcagc tgctctccgc
      361 ctgatgaaaa tcccgctggt tgtatatggc tgtcagaatg aacgatttgg tggttgtggc
      421 tctgttctaa atattgcctc tgctgaccta ccaaacactg ggagaccatt tcagtgtatc
      481 cctggatatc gggctgagga agcagtggaa atgttaaaga ccttctacaa acaagaaaat
      541 ccaaatgcac caaaatcgaa agttcggaaa tgacagtaaa ggtggatacg gatccgaa
//
