LOCUS       sequence-57243-e         829 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 829)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: DDX52A
FEATURES             Location/Qualifiers
     source          1..829
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaagataaa cttcttgcgg aataaacaca aaattcacgt ccaaggaacc
      121 gatcttcctg acccaattgc tacatttcag caacttgacc aggaatataa aatcaattct
      181 cgactacttc agaacattct agatgcaggt ttccaaatgc ctacgccaat ccaaatgcaa
      241 gccatcccag ttatgctgca tggtcgggaa cttctggctt ctgctccaac tggatctgga
      301 aaaacattag cttttagcat tcctatttta atgcagctga aacaacccgc aaataaaggc
      361 ttcagagccc tgattatatc accaacacga gaacttgcca gccagattca cagagagtta
      421 ataaaaattt ctgagggaac aggattcagg atacacatga tccacaaagc agcagtggca
      481 gccaagaaat ttggacctaa atcatctaaa aagtttgata ttcttgtgac tactccaaat
      541 cgactaatct atttattaaa gcaagatccc cccggaatcg acctagcaag tgttgagtgg
      601 cttgtagtag acgaatcaga taaactgttt gaagatggca aaactgggtt cagagaccag
      661 ctggcttcca ttttcctggc ctgcacatcc cacaaggtcc gaagagctat gttcagtgca
      721 acttttgcat atgatgttga acagtggtgc aaactcaacc tggacaatgt catcagtgtg
      781 tccattggag caaggaattc ttgacagtaa aggtggatac ggatccgaa
//
