LOCUS       sequence-57245-e        1312 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1312)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1312)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ATTYA
FEATURES             Location/Qualifiers
     source          1..1312
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgtggtctgt gaggccctca gacatggcca agaaaacttt caaccccatc
      121 cgagccattg tggacaacat gaaggtgaaa ccaaatccaa acaaaaccat gatttccctg
      181 tccattgggg accctactgt gtttggaaac ctgcctacag accctgaagt tacccaggca
      241 atgaaagatg ccctggactc gggcaaatat aatggctatg ccccatccat cggcttccta
      301 tccagtcggg aggagattgc ttcttattac cactgtcctg aggcacccct agaagctaag
      361 gacgtcattc tgacaagtgg ctgcagccaa gctattgacc tttgtttagc tgtgttggcc
      421 aacccagggc aaaacatcct ggttccaaga cctggtttct ctctctacaa gactctggct
      481 gagtctatgg gaattgaggt caaactctac aatttgttgc cagagaaatc ttgggaaatt
      541 gacctgaaac aactggaata tctaattgat gaaaagacag cttgtctcat tgtcaataat
      601 ccatcaaacc cctgtgggtc agtgttcagc aaacgtcatc ttcagaagat tctggcagtg
      661 gctgcacggc agtgtgtccc catcttagct gatgagatct atggagacat ggtgttttcg
      721 gattgcaaat atgaaccact ggccaccctc agcaccgatg tccccatcct gtcctgtgga
      781 gggctggcca agcgctggct ggttcctggc tggaggttgg gctggatcct cattcatgac
      841 cgaagagaca tttttggcaa tgagatccga gatgggctgg tgaagctgag tcagcgcatt
      901 ttgggaccct gtaccattgt ccagggagct ctgaaaagca tcctatgtcg caccccggga
      961 gagttttacc acaacactct gagcttcctc aagtccaatg ctgatctctg ttatggggcg
     1021 ttggctgcca tccctggact ccggccagtc cgcccttctg gggctatgta cctcatggtt
     1081 ggaattgaga tggaacattt cccagaattt gagaacgatg tggagttcac ggagcggtta
     1141 gttgctgagc agtctgtcca ctgcctccca gcaacgtgct ttgagtaccc gaatttcatc
     1201 cgagtggtca tcacagtccc cgaggtgatg atgctggagg cgtgcagccg gatccaggag
     1261 ttctgtgagc agcactacca ttgttgacag taaaggtgga tacggatccg aa
//
