LOCUS       sequence-57246-e        1162 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1162)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SERCA
FEATURES             Location/Qualifiers
     source          1..1162
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgctgccgca ctcagtgttg ttagagatac aaaaggaatt attagactac
      121 aaaggagttg gcattagtgt tcttgaaatg agtcacaggt catcagattt tgccaagatt
      181 attaacaata cagagaatct tgtgcgggaa ttgctagctg ttccagacaa ctataaggtg
      241 atttttctgc aaggaggtgg gtgcggccag ttcagtgctg tccccttaaa cctcattggc
      301 ttgaaagcag gaaggtgtgc ggactatgtg gtgacaggag cttggtcagc taaggccgca
      361 gaagaagcca agaagtttgg gactataaat atcgttcacc ctaaacttgg gagttataca
      421 aaaattccag atccaagcac ctggaacctc aacccagatg cctcctacgt gtattattgc
      481 gcaaatgaga cggtgcatgg tgtggagttt gactttatac ccgatgtcaa gggagcagta
      541 ctggtttgtg acatgtcctc aaacttcctg tccaagccag tggatgtttc caagtttggt
      601 gtgatttttg ctggtgccca gaagaatgtt ggctctgctg gggtcaccgt ggtgattgtc
      661 cgtgatgacc tgctggggtt tgccctccga gagtgcccct cggtcctgga atacaaggtg
      721 caggctggaa acagctcctt gtacaacacg cctccatgtt tcagcatcta cgtcatgggc
      781 ttggttctgg agtggattaa aaacaatgga ggtgccgcgg ccatggagaa gcttagctcc
      841 atcaaatctc aaacaattta tgagattatt gataattctc aaggattcta cgtttgtcca
      901 gtggagcccc aaaatagaag caagatgaat attccattcc gcattggcaa tgccaaagga
      961 gatgatgctt tagaaaaaag atttcttgat aaagctcttg aactcaatat gttgtccttg
     1021 aaagggcata ggtctgtggg aggcatccgg gcctctctgt ataatgctgt cacaattgaa
     1081 gacgttcaga agctggccgc cttcatgaaa aaatttttgg agatgcatca gctatgacag
     1141 taaaggtgga tacggatccg aa
//
