LOCUS       sequence-57248-e        1117 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1117)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PCYT2A
FEATURES             Location/Qualifiers
     source          1..1117
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggggggcag gcgcgccgtg agggtgtggt gcgatggctg ctatgacatg
      121 gtgcattacg gccactccaa ccagctgcgc caggcacggg ccatgggtga ctacctcatc
      181 gtaggcgtgc acaccgatga ggagatcgcc aagcacaagg ggcccccggt gttcactcag
      241 gaggagagat acaagatggt gcaggccatc aaatgggtgg acgaggtggt gccagcggct
      301 ccctacgtca ctacactaga gaccctggac aaatacaact gtgacttctg tgttcacggc
      361 aatgacatca ccctgactgt agatggccgg gacacctatg aggaagtaaa gcaggctggg
      421 aggtacagag aatgcaagcg cacgcaaggg gtgtccacca cagacctcgt gggccgcatg
      481 ctgctggtaa ccaaagccca tcacagcagc caggagatgt cctctgagta ccgggagtat
      541 gcagacagtt ttggcaagtg ccctggtggg cggaacccct ggaccggggt atcccagttc
      601 ctgcagacat ctcagaagat catccagttt gcttctggga aggagcccca gccaggggag
      661 acagtcatct atgtggctgg tgccttcgac ctgttccaca tcgggcatgt ggacttcctg
      721 gagaaggtgc acaggctggc agagaggccc tacatcatcg cgggcttaca ctttgaccag
      781 gaggtcaatc actacaaggg gaagaactac cccatcatga atctgcatga acggactctg
      841 agcgtgctgg cctgccggta cgtgtcagaa gtggtgattg gagccccgta cgcggtcaca
      901 gcagagctcc taagtcactt caaggtggac ctggtgtgtc acggcaagac agaaattatc
      961 cctgacaggg atggctccga cccataccag gagcccaaga gaaggggcat cttccgtcag
     1021 attgacagtg gcagcaacct caccacagac ctcatcgtcc agcggatcat caccaacagg
     1081 ttggagtatt gacagtaaag gtggatacgg atccgaa
//
