LOCUS       sequence-57249-e         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: CTPS2A
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgaaaatatg ctccatagcc ctggttggca aatacaccaa gctcagagac
      121 tgctacgcct ctgtgttcaa agccctggaa cactcagccc tggccatcaa ccacaagttg
      181 aatctgatgt acatagactc cattgatctg gagaagatca ctgaaaccga ggaccctgtg
      241 aaatttcatg aagcttggca gaagctatgc aaagctgatg gtattcttgt gcctggaggc
      301 tttggaatca gaggaacatt gggaaaactc caggcgattt cttgggcaag gacaaagaag
      361 attccttttc tgggagtttg tcttgggatg caactagcag tgatagagtt tgcaagaaac
      421 tgccttaact tgaaagatgc tgattccaca gagtttaggc caaatgcccc agttcctctg
      481 gtgattgata tgcccgagca caaccctggc aatttgggag gaacaatgag actgggaata
      541 agaagaactg ttttcaaaac tgaaaattca atattaagga aactttatgg tgatgttcct
      601 tttatagaag aaagacacag acatcggttc gaggtaaacc ctaacctgat caaacaattt
      661 gagcagaatg acttaagttt tgtaggtcag gatgttgatg gagacaggat ggaaatcatt
      721 gaactggcaa atcatcctta ttttgttggt gtccagttcc atcctgagtt ttcttctagg
      781 ccgatgaagc cttcccctcc gtatctgggg ctgttacttg cagcaactgg gaacctgaat
      841 gcctacttgc aacagggttg caaactgtct taacagtaaa ggtggatacg gatccgaa
//
