LOCUS       sequence-57250-e         406 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 406)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 406)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SKILA
FEATURES             Location/Qualifiers
     source          1..406
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgccttcaga tagctccaca gaactcactc agactgtgtt ggaaggggaa
      121 tctatttctt gttttcaagt tggaggagaa aagagactct gtttgcccca agtcttaaat
      181 tctgttctcc gagaatttac actccagcaa ataaatacag tgtgtgatga actgtacata
      241 tattgttcaa ggtgtacttc agaccagctt catatcttaa aggtactggg catacttcca
      301 ttcaatgccc catcctgtgg gctgattaca ttaactgatg cacaaagatt atgtaatgct
      361 ttattgcggc cacgaacttg acagtaaagg tggatacgga tccgaa
//
