LOCUS       sequence-57252-e         535 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 535)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 535)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NUMBLA
FEATURES             Location/Qualifiers
     source          1..535
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggctagccg gccacaccaa tggcaagctg acgaagatgc tgttcggaag
      121 ggtacctgct cctttccggt ccgctacctg ggtcatgtag aggtggagga atcgcggggc
      181 atgcatgttt gtgaggacgc agtcaaaaag ttaaaagcca tggggcgtaa gtcagtcaaa
      241 agtgtgctgt gggtttcagc ggatggcctg cgcgtcgtgg acgataaaac caaagattta
      301 ctggtagacc agacgattga aaaggtttcc ttctgcgccc cagatcgtaa cctggacaaa
      361 gcattttcgt atatctgtcg cgatggtact acacggcgtt ggatttgcca ctgtttctta
      421 gctctgaaag acagcgggga acgcctgtct cacgcggtgg gatgcgcctt tgcagcgtgt
      481 ttagagcgta agcagcgtcg cgaaaaatga cagtaaaggt ggatacggat ccgaa
//
