LOCUS       sequence-57256-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT11A
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcggcccca gcccggcgat ggtgagcggc ggtaccgaga ggccagtgcc
      121 agaaagaaga tccgcctgga caggaagtac atcgtgtcct gtaagcagac agaggtgccc
      181 ctctccgtcc cctgggaccc tagcaaccag gtgtacctga gctacaataa cgtctcctcc
      241 ttgaagatgc ttgtggccaa ggacaactgg gtgctgtcct cggagatcag tcaggtccgc
      301 ctgtacactc tggaggatga caagttcctc tccttccaca tggagatggt ggtgcatgtg
      361 gatgcagccc aggccttcct gctgctctcg gacctgcgtc agaggccaga gtgggacaag
      421 cactaccgga gcgtggagct agtgcagcag gtagacgagg acgacgccat ctaccacgtc
      481 accagccctg ccctcggagg tcacacaaag ccccaggact tcgtgatcct ggcctcgagg
      541 cggaagcctt gtgacaatgg ggacccctat gtcatcgcgc tgaggtcggt cacgctgccc
      601 acacaccgag agacgccaga gtacagacgc ggagagaccc tctgctcagg cttctgcctc
      661 tggcgcgagg gggaccagct gaccaaggta tcctactaca accaggccac cccaggtgtt
      721 ctcaactatg tgaccaccaa cgtggccggc ctctcctctg agttctacac caccttcaag
      781 gcttgtgagc agtttctctt ggacaaccgg aatgatctgg cccccagcct ccagaccctc
      841 tgacagtaaa ggtggatacg gatccgaa
//
