LOCUS       sequence-57260-e        1390 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1390)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: LMSAHA
FEATURES             Location/Qualifiers
     source          1..1390
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggcggacta caaggtaaaa gacatcagcc tcgcggagtg gggccgcaag
      121 gcgatcgagc tcgcggaaaa cgagatgccc ggtctgatgg agctgcgccg cgagtacgga
      181 ccctcccagc cgctgaaggg cgccaagatt gccggctgcc tgcacatgac cgtacagact
      241 gccgtgctga tcgagaccct caaggccctc ggtgcggagc tgcgctggtc gtcgtgcaac
      301 atcttttcca ctcaggataa cgccgctgcg gccattgcca agactggcgt accggtgttt
      361 gcgtggaagg gtgagacaga cgaggagtat gagtggtgca tcgcccagac agtgaagggc
      421 ttcagcggtg acggtctgcc gaacatgatc ctcgacgacg gtggcgacct cacgaatctg
      481 gtgatcgacc gctaccccga gctcgtgccc aagatcttcg gtatctccga ggagaccacg
      541 acgggtgtga agaacctgta caagcgcctg agcaagggca acctccccat ctccgccatc
      601 aacgttaacg acagcgtgac gaagagcaag ttcgacaacc tttacggctg ccgagagtcc
      661 ttggtggacg gcatcaagcg cgccacggat gtcatgattg ccggcaagac gtgctgcgtg
      721 tgcggatacg gtgatgttgg taagggctgc gccgccgccc tgcgcgcctt cggcgctcgc
      781 gttgtggtaa cggaggtgga ccctatcaat gcccttcagg cctccatgga gggctaccag
      841 gtcgctctgg tcgaagacgt catggccgat gcgcacatct tcgtgacgac caccggcaac
      901 gatgacatca tcacctctga ccacttccct cacatgcgcg acgacgccat tgtgtgcaac
      961 atcggccact tcgacacgga gatccaggtg ggatggcttg aggcaaacgc caaagagcac
     1021 gtggaaatca agcctcaggt ggaccgctat accatggaga acggccgtca catcatcctg
     1081 ctggctaagg gccgcctggt caacctcggc tgcgccagcg gtcacccgtc cttcgtgatg
     1141 tccaactcct tcacgaacca ggtgctggct cagatcgagc tctggagcaa ccgcgacaac
     1201 ggcaagtacc cgcgcggtga caaggccggc gtgttcttcc tgcccaaggc gctcgatgag
     1261 aaggtcgccg ccctccacct cgcccacgtc ggcgccaagc tgacaaaact gaccccgaag
     1321 caggccgagt acatcaactg cccggtcaac ggcccgttca agtgacagta aaggtggata
     1381 cggatccgaa
//
