LOCUS       sequence-57268-e        1402 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1402)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: TBSAHA
FEATURES             Location/Qualifiers
     source          1..1402
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgtacaaggt acgtgatatc agcctcgctg aatggggccg cagggagctt
      121 gagctcgcag agaacgaaat gccgggtctc atggaacttc gccgtgagta cggcccttcg
      181 aagcctctga agggtgccaa gatcgctggc tgcctgcaca tgactatgca gactgctgtg
      241 cttatcgaga cgcttgtgga gctcggtgct gaagtgcggt gggcctcgtg caacatcttc
      301 tccacacagg accatgcagc cgccgcgatt gcgaagcggg gtatccctgt ttttgcgtgg
      361 aagggcgaaa ctgaagagga gtacatgtgg tgtatgaagc agacactgaa gggcttcagc
      421 ggtgatggtt accccaacat gttgctcgac gacggtggcg acctgacaaa ttacgtcctg
      481 gatgagtgca aggagctgga cggaaaaata tacggtgtct ccgaggagac aacgacaggt
      541 gtaaaaaacc tgtacaagcg gctgcagcgg ggtaagctga ccatccccgc catgaatgtg
      601 aacgacagtg tgacgaagag caagttcgac aatctgtacg gttgccgtga gtctctcgtg
      661 gacggcatca agcgtgctac cgatgtaatg attgctggca agaccgcatg tgtctgtgga
      721 tatggtgacg tgggcaaagg ttgcgcggct gcgctgcgtg gctttggagc acgtgtcgtt
      781 gtgacggagg ttgatccaat taatgcgctg caggctgcga tggagggcta tcaggtgcta
      841 ttggttgagg atgttgtcga ggaggcacac attttcgtca cgacgaccgg caacgacgat
      901 atcatcacat cagagcattt cccccgcatg cgtgacgatg caattgtgtg caacatcggc
      961 cacttcgaca cagagatcca ggtggcgtgg ctgaaggcaa atgcgaagga gcgcgtggaa
     1021 gtgaaaccgc aggtggatcg ctacaccatg gccaatggcc gccacattat tcttctggct
     1081 gagggtcgcc tggtcaacct gggttgtgct tctggccatc cctcctttgt gatgtcgaac
     1141 tccttctgca accaggtcct tgcccaaatt gagttgtgga caaaccgaga caccggcaag
     1201 tacccacgtg gcgccaaggc tcaggtttac ttcctaccca agaagcttga cgaaaaggtt
     1261 gctgcattgc acctcggcaa gctgggcgct aagctgacaa agctcacacc gaaacaggca
     1321 gaatacatca actgtcctgt cgacggtccc ttcaaacctg accactaccg ttactgacag
     1381 taaaggtgga tacggatccg aa
//
