LOCUS       sequence-57276-e        1894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1894)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIK3C3A
FEATURES             Location/Qualifiers
     source          1..1894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgtctgacca cgatctgaaa cccaatgctg ccacgagaga tcagttaaat
      121 attattgtga gttatccacc aaccaagcaa cttacatatg aagaacaaga tcttgtttgg
      181 aagtttagat attatcttac gaatcaagaa aaagccttga caaaattctt gaaatgtgtt
      241 aattgggatc tacctcaaga ggccaaacag gccttggaac ttctgggaaa atggaagccg
      301 atggatgtag aggactcctt ggagctgtta tcctctcatt acaccaaccc aactgtgagg
      361 cgttatgctg ttgcccggtt gcgacaggcc gatgatgagg atttgttgat gtacctatta
      421 caattggtcc aggctctcaa atatgaaaat tttgatgata taaagaatgg attggaacct
      481 accaagaagg atagtcagag ttcagtgtca gaaaatgtgt caaattctgg aataaattct
      541 gcagaaatag atagctccca aattataacc agcccccttc cttcagtctc ttcacctcct
      601 cctgcatcaa aaacaaaaga agttccagat ggcgaaaatc tggaacaaga tctctgtacc
      661 ttcttgatat cgagagcctg caaaaactca acactggcta attatttata ctggtatgtg
      721 atagtggaat gtgaagatca agatactcag cagagagatc caaagaccca tgagatgtac
      781 ttgaacgtaa tgagaagatt cagccaagca ttgttgaagg gtgataagtc tgtcagagtt
      841 atgcgttctt tgctggctgc acaacagaca tttgtagatc ggttggtgca tctaatgaag
      901 gcagtacaac gcgaaagtgg aaatcgtaag aaaaagaatg agagactaca ggcattgctt
      961 ggagataatg aaaagatgaa tttgtcagat gtggaactta tcccgttgcc tttagaaccc
     1021 caagtgaaaa ttagaggaat aattccggaa acagctacac tgtttaaaag tgcccttatg
     1081 cctgcacagt tgttttttaa gacggaagat ggaggcaaat atccagttat atttaagcat
     1141 ggagatgatt tacgtcaaga tcaacttatt cttcaaatca tttcactcat ggacaagctg
     1201 ttacggaaag aaaatctgga cttgaaattg acaccttata aggtgttagc caccagtaca
     1261 aaacatggct tcatgcagtt tatccagtca gttcctgtgg ctgaagttct tgatacagag
     1321 ggaagcattc agaacttttt tagaaaatat gcaccaagtg agaatgggcc aaatgggatt
     1381 agtgctgagg tcatggacac ttacgttaaa agctgtgctg gatattgcgt gatcacctat
     1441 atacttggag ttggagacag gcacctggat aaccttttgc taacaaaaac aggcaaactc
     1501 ttccacatag actttggata tattttgggt cgggatccaa agcctcttcc tccaccaatg
     1561 aagctgaata aagaaatggt agaaggaatg gggggcacac agagtgagca gtaccaagag
     1621 ttccgtaaac agtgttacac ggctttcctc cacctgcgaa ggtattctaa tctgattttg
     1681 aacttgtttt ccttgatggt tgatgcaaac attccagata ttgcacttga accagataaa
     1741 actgtgaaaa aggttcagga taaattccgc ttagacctgt cggatgaaga ggctgtgcat
     1801 tacatgcaga gtctgattga tgagagtgtc catgctcttt ttgctgcagt ggtggaacag
     1861 attcactgac agtaaaggtg gatacggatc cgaa
//
