LOCUS       sequence-57279-e        1255 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1255)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: HSPA5A
FEATURES             Location/Qualifiers
     source          1..1255
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggacgtggg cacggtggtc ggcatcgacc tggggaccac ctactcctgc
      121 gtcggcgtgt tcaagaacgg ccgcgtggag atcatcgcca acgatcaggg caaccgcatc
      181 acgccgtcct atgtcgcctt cactcctgaa ggggaacgtc tgattggcga tgccgccaag
      241 aaccagctca cctccaaccc cgagaacacg gtctttgacg ccaagcggct catcggccgc
      301 acgtggaatg acccgtctgt gcagcaggac atcaagttct tgccgttcaa ggtggttgaa
      361 aagaaaacta aaccatacat tcaagttgat attggaggtg ggcaaacaaa gacatttgct
      421 cctgaagaaa tttctgccat ggttctcact aaaatgaaag aaaccgctga ggcttatttg
      481 ggaaagaagg ttacccatgc agttgttact gtaccagcct attttaatga tgcccaacgc
      541 caagcaacca aagacgctgg aactattgct ggcctaaatg ttatgaggat catcaacgag
      601 cctacggcag ctgctattgc ttatggcctg gataagaggg agggggagaa gaacatcctg
      661 gtgtttgacc tgggtggcgg aaccttcgat gtgtctcttc tcaccattga caatggtgtc
      721 ttcgaagttg tggccactaa tggagatact catctgggtg gagaagactt tgaccagcgt
      781 gtcatggaac acttcatcaa actgtacaaa aagaagacgg gcaaagatgt caggaaagac
      841 aatagagctg tgcagaaact ccggcgcgag gtagaaaagg ccaaacgggc cctgtcttct
      901 cagcatcaag caagaattga aattgagtcc ttctatgaag gagaagactt ttctgagacc
      961 ctgactcggg ccaaatttga agagctcaac atggatctgt tccggtctac tatgaagccc
     1021 gtccagaaag tgttggaaga ttctgatttg aagaagtctg atattgatga aattgttctt
     1081 gttggtggct cgactcgaat tccaaagatt cagcaactgg ttaaagagtt cttcaatggc
     1141 aaggaaccat cccgtggcat aaacccagat gaagctgtag cgtatggtgc tgctgtccag
     1201 gctggtgtgc tctctggtga tcaagattga cagtaaaggt ggatacggat ccgaa
//
