LOCUS       sequence-57281-e        1360 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1360)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: ACOT4A
FEATURES             Location/Qualifiers
     source          1..1360
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgtcagcaac gctgatcctg gagcccccag gccgctgctg ctggaacgag
      121 ccggtgcgca ttgccgtgcg cggcctggcc ccggagcagc gggttacgct gcgcgcgtcc
      181 ctgcgcgacg agaagggcgc gctcttccgg gcccacgcgc gctactgcgc cgacgcctgc
      241 ggcgagctgg acctggagcg cgcacccgcg ctgggcggca gcttcgcggg actcgagccc
      301 atggggctgc tctgggccct ggaacccgag aagccttttt ggcgcttcct gaagcgggac
      361 gtacagattc cttttgtcgt ggagttggag gtgctggacg gccacgaccc cgagcctgga
      421 cggctgctgt gccaggcgca gcacgagcgc cacttcctcc cgccaggggt gcggcgccag
      481 tcggtgcgag cgggccgggt gcgcgccacg ctcttcctgc cgccaggacc tggacccttc
      541 ccagggatca ttgacatctt tggtattgga gggggcctct tggaatatcg agccagcctc
      601 cttgctggcc atggctttgc cacgttggct ctagcttatt ataactttga agatctcccc
      661 aataacatgg acaacatatc cctggagtac ttcgaagaag ccgtatgcta catgcttcaa
      721 catccccagg taaaaggccc aggcattggg cttttgggca tttctctagg agctgatatt
      781 tgtctctcaa tggcctcatt cttgaagaat gtctcagcca cagtttccat caatggatct
      841 gggatcagtg ggaacacagc catcaactat aagcacagta gcattccacc attgggctat
      901 gacctgagga gaatcaaggt agctttctca ggcctcgtgg acatcgtgga tataaggaat
      961 gctctcgtag gagggtacaa gaaccccagc atgattccaa tagagaaggc ccaggggccc
     1021 atcctgctca ttgttggtca ggatgaccat aactggagaa gtgagttgta tgcccaaaca
     1081 gtctctgaac ggttacaggc ccatggaaag gaaaaacccc agatcatctg ttaccctggg
     1141 actgggcatt acatcgagcc tccttacttc cccctgtgcc cagcttccct tcacagatta
     1201 ctgaacaaac atgttatatg gggtggggag cccagggctc attctaaggc ccaggaagat
     1261 gcctggaagc aaattctagc cttcttctgc aaacacctgg gaggtaccca gaaaacagct
     1321 gtccctaaat tgtgacagta aaggtggata cggatccgaa
//
