LOCUS       sequence-57282-e        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PARP2A
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(713..724)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggatcttcg ggtacaggag ttaataaagt tgatctgtaa tgttcaggcc
      121 atggaagaaa tgatgatgga aatgaagtat aataccaaga aagccccact tgggaagctg
      181 acagtggcac aaatcaaggc aggttaccag tctcttaaga agattgagga ttgtattcgg
      241 gctggccagc atggacgagc tctcatggaa gcatgcaatg aattctacac caggattccg
      301 catgactttg gactccgtac tcctccacta atccggacac agaaggaact gtcagaaaaa
      361 atacaattac tagaggcttt gggagacatt gaaattgcta ttaagctggt gaaaacagag
      421 ctacaaagcc cagaacaccc attggaccaa cactatagaa acctacattg tgccttgcgc
      481 ccccttgacc atgaaagtta tgagttcaaa gtgatttccc agtacctaca atctacccat
      541 gctcccacac acagcgacta taccatgacc ttgctggatt tgtttgaagt ggagaaggat
      601 ggtgagaaag aagccttcag agaggacctt cataacagga tgcttctatg gcatggttcc
      661 aggatgagta actgggtggg aatcttgagc catgggcttc gaattgccca ccctgaagct
      721 cccatcacag gttacatgtt tgggaaagga atctactttg ctgacatgtc ttccaagagt
      781 gccaattact gctttgcctc tcgcctaaag aatacaggac tgctgctctt atcagaggta
      841 gctctaggtc agtgtaatga actactagag gccaatccta aggccgaagg attgcttcaa
      901 ggtaaacata gcaccaaggg gctgggcaag atggctccca gttctgccca cttcgtcacc
      961 ctgaatggga gtacagtgcc attaggacca gcaagtgaca caggaattct gaatccagat
     1021 ggttataccc tcaactacaa tgaatatatt gtatataacc ccaaccaggt ccgtatgcgg
     1081 taccttttaa aggttcagtt taatttctga cagtaaaggt ggatacggat ccgaa
//
