LOCUS       sequence-57290-e        1936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1936)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIK3C3A
FEATURES             Location/Qualifiers
     source          1..1936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgagcaaaca ccacaagctt gcccggagtt taagaagtgg accttctgac
      121 cacgatctga aacccaatgc tgccacgaga gatcagttaa atattattgt gagttatcca
      181 ccaaccaagc aacttacata tgaagaacaa gatcttgttt ggaagtttag atattatctt
      241 acgaatcaag aaaaagcctt gacaaaattc ttgaaatgtg ttaattggga tctacctcaa
      301 gaggccaaac aggccttgga acttctggga aaatggaagc cgatggatgt agaggactcc
      361 ttggagctgt tatcctctca ttacaccaac ccaactgtga ggcgttatgc tgttgcccgg
      421 ttgcgacagg ccgatgatga ggatttgttg atgtacctat tacaattggt ccaggctctc
      481 aaatatgaaa attttgatga tataaagaat ggattggaac ctaccaagaa ggatagtcag
      541 agttcagtgt cagaaaatgt gtcaaattct ggaataaatt ctgcagaaat agatagctcc
      601 caaattataa ccagccccct tccttcagtc tcttcacctc ctcctgcatc aaaaacaaaa
      661 gaagttccag atggcgaaaa tctggaacaa gatctctgta ccttcttgat atcgagagcc
      721 tgcaaaaact caacactggc taattattta tactggtatg tgatagtgga atgtgaagat
      781 caagatactc agcagagaga tccaaagacc catgagatgt acttgaacgt aatgagaaga
      841 ttcagccaag cattgttgaa gggtgataag tctgtcagag ttatgcgttc tttgctggct
      901 gcacaacaga catttgtaga tcggttggtg catctaatga aggcagtaca acgcgaaagt
      961 ggaaatcgta agaaaaagaa tgagagacta caggcattgc ttggagataa tgaaaagatg
     1021 aatttgtcag atgtggaact tatcccgttg cctttagaac cccaagtgaa aattagagga
     1081 ataattccgg aaacagctac actgtttaaa agtgccctta tgcctgcaca gttgtttttt
     1141 aagacggaag atggaggcaa atatccagtt atatttaagc atggagatga tttacgtcaa
     1201 gatcaactta ttcttcaaat catttcactc atggacaagc tgttacggaa agaaaatctg
     1261 gacttgaaat tgacacctta taaggtgtta gccaccagta caaaacatgg cttcatgcag
     1321 tttatccagt cagttcctgt ggctgaagtt cttgatacag agggaagcat tcagaacttt
     1381 tttagaaaat atgcaccaag tgagaatggg ccaaatggga ttagtgctga ggtcatggac
     1441 acttacgtta aaagctgtgc tggatattgc gtgatcacct atatacttgg agttggagac
     1501 aggcacctgg ataacctttt gctaacaaaa acaggcaaac tcttccacat agactttgga
     1561 tatattttgg gtcgggatcc aaagcctctt cctccaccaa tgaagctgaa taaagaaatg
     1621 gtagaaggaa tggggggcac acagagtgag cagtaccaag agttccgtaa acagtgttac
     1681 acggctttcc tccacctgcg aaggtattct aatctgattt tgaacttgtt ttccttgatg
     1741 gttgatgcaa acattccaga tattgcactt gaaccagata aaactgtgaa aaaggttcag
     1801 gataaattcc gcttagacct gtcggatgaa gaggctgtgc attacatgca gagtctgatt
     1861 gatgagagtg tccatgctct ttttgctgca gtggtggaac agattcactg acagtaaagg
     1921 tggatacgga tccgaa
//
