LOCUS       sequence-57292-e         823 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 823)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 823)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBF1A
FEATURES             Location/Qualifiers
     source          1..823
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgcggagtgg aagcagcatg aaggaagagc cgctgggcag cggcatgaac
      121 gcggtgcgga cgtggatgca gggcgccggg gtgctggacg ccaacacggc ggcgcagagc
      181 ggggtgggtc tggcccgggc tcactttgag aagcagccgc cttccaatct gcggaaatcc
      241 aacttcttcc acttcgtcct ggccctctac gacagacagg gccagcccgt ggagatcgag
      301 aggacagcgt ttgtggggtt cgtggagaag gaaaaagaag ccaacagcga aaagaccaat
      361 aacggaattc actaccggct tcagcttctc tacagcaatg ggataaggac ggagcaggat
      421 ttctacgtgc gcctcattga ctccatgaca aaacaagcca tagtgtatga aggccaagac
      481 aagaacccag aaatgtgccg agtcttgctc acacatgaga tcatgtgcag ccgctgttgt
      541 gacaagaaaa gctgtggcaa ccgaaatgag actccctcag atccagtgat aattgacagg
      601 ttcttcttga aatttttcct caaatgtaac caaaattgcc taaagaatgc gggaaaccca
      661 cgtgacatgc ggagattcca ggtcgtggtg tctacgacag tcaatgtgga tggccatgtc
      721 ctggcagtct ctgataacat gtttgtccat aataattcca agcatgggcg gagggctcgg
      781 aggcttgacc cctcgtgaca gtaaaggtgg atacggatcc gaa
//
