LOCUS       sequence-57293-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NVLA
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgactgtccc taatgtgaca tgggcagata ttggtgccct ggaagacatt
      121 agagaggagc tcaccatggc aatattggca ccagtacgca acccagacca gttcaaagct
      181 cttggattgg tgactccagc tggggtcctc cttgctggtc ctcctggctg tgggaagact
      241 ctgctggcga aggctgttgc aaatgagtcc ggactaaatt ttatatctgt caagggcccc
      301 gaattactaa acatgtatgt tggtgagagt gaacgtgctg tgcgacaagt ttttcaacga
      361 gccaagaact cagcaccctg tgtgatattc tttgatgaag tggatgcttt atgtcctcga
      421 agatcagacc gagagacagg ggcaagtgtc cgagtggtga atcagctact tacagagatg
      481 gatggtctgg aagcacgcca gcaggttttt attatggcag ccactaacag gccagatata
      541 attgaccctg caatcctgcg cccgggccgc ctggacaaaa cactgtttgt gggtttaccg
      601 ccccctgcag atcgccttgc catcttaaaa actatcacaa aaaatggtac caaaccacca
      661 ctggatgcag atgtaaattt ggaagcaatt gctggtgacc ttcgctgtga ttgctatacg
      721 ggcgcagatc tctctgcttt ggtacgagaa gcttctatct gtgccctgag acaggaaatg
      781 gcaagacaga agagtggaaa tgaaaaaggt gaactcaagg ttagtcataa gcattttgaa
      841 gaagctttca agaaagtaag atcatctata tcaaaaaagg atcaaatctg acagtaaagg
      901 tggatacgga tccgaa
//
