LOCUS       sequence-57297-e        1441 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1441)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AHCYL1A
FEATURES             Location/Qualifiers
     source          1..1441
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             34..57
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             64..84
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggttggtccc atccgcaatt tgaaaagggt
       61 accgagaacc tgtacttcca atccatggtt tctccccgag agaagcagca aaccaactcc
      121 aagggcagca gcaatttctg tgtgaagaac atcaagcagg cagaatttgg acgccgggag
      181 attgagattg cagagcaaga catgtctgct ctgatttcac tcaggaaacg tgctcagggg
      241 gagaagccct tggctggtgc taaaatagtg ggctgtacac acatcacagc ccagacagcg
      301 gtgttgattg agacactctg tgccctgggg gctcagtgcc gctggtctgc ttgtaacatc
      361 tactcaactc agaatgaagt agctgcagca ctggctgagg ctggagttgc agtgttcgct
      421 tggaagggcg agtcagaaga tgacttctgg tggtgtattg accgctgtgt gaacatggat
      481 gggtggcagg ccaacatgat cctggatgat gggggagact taacccactg ggtttataag
      541 aagtatccaa acgtgtttaa gaagatccga ggcattgtgg aagagagcgt gactggtgtt
      601 cacaggctgt atcagctctc caaagctggg aagctctgtg ttccggccat gaacgtcaat
      661 gattctgtta ccaaacagaa gtttgataac ttgtactgct gccgagaatc cattttggat
      721 ggcctgaaga ggaccacaga tgtgatgttt ggtgggaaac aagtggtggt gtgtggctat
      781 ggtgaggtag gcaagggctg ctgtgctgct ctcaaagctc ttggagcaat tgtctacatt
      841 accgaaatcg accccatctg tgctctgcag gcctgcatgg atgggttcag ggtggtaaag
      901 ctaaatgaag tcatccggca agtcgatgtc gtaataactt gcacaggaaa taagaatgta
      961 gtgacacggg agcacttgga tcgcatgaaa aacagttgta tcgtatgcaa tatgggccac
     1021 tccaacacag aaatcgatgt gaccagcctc cgcactccgg agctgacgtg ggagcgagta
     1081 cgttctcagg tggaccatgt catctggcca gatggcaaac gagttgtcct cctggcagag
     1141 ggtcgtctac tcaatttgag ctgctccaca gttcccacct ttgttctgtc catcacagcc
     1201 acaacacagg ctttggcact gatagaactc tataatgcac ccgaggggcg atacaagcag
     1261 gatgtgtact tgcttcctaa gaaaatggat gaatacgttg ccagcttgca tctgccatca
     1321 tttgatgccc accttacaga gctgacagat gaccaagcaa aatatctggg actcaacaaa
     1381 aatgggccat tcaaacctaa ttattacaga tactgacagt aaaggtggat acggatccga
     1441 a
//
