LOCUS       sequence-57300-e         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: EBF3A
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgtcagaagc cactccgtgc atcaaggcca tcagtcccag tgaaggctgg
      121 accacggggg gtgccaccgt catcataatt ggcgacaact tctttgacgg gctgcaagtt
      181 gtattcggaa ctatgttggt gtggagcgag ctgataactc cccatgccat ccgagtccag
      241 accccgccga ggcacattcc tggcgtcgtc gaagtgaccc tctcctacaa atccaagcag
      301 ttctgcaaag gtgctcctgg gcgctttgtc tacaccgccc ttaatgaacc aaccatagat
      361 tacggctttc agaggttgca gaaagtgatc ccaagacatc cgggtgatcc cgaaaggtta
      421 cccaaggagg tgttactgaa gcgggcggcg gacctggtgg aagccttata cggaatgcct
      481 cacaacaacc aggagatcat cttgaagcga gcggcggaca tcgccgaggc gctgtacagc
      541 gtttgacagt aaaggtggat acggatccga a
//
