LOCUS       sequence-57305-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MPSTA
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggtgtcggc gcaatgggtg gcggaggcgc tgcgggcccc gcgcgctggg
      121 cagcctctgc agctgctgga cgcctcctgg tacctgccga agctggggcg cgacgcgcga
      181 cgcgagttcg aggagcgcca catcccgggc gccgctttct tcgacatcga ccagtgcagc
      241 gaccgcacct cgccctacga ccacatgctg cccggggccg agcatttcgc ggagtacgca
      301 ggccgcctgg gcgtgggcgc ggccacccac gtcgtgatct acgacgccag cgaccagggc
      361 ctctactccg ccccgcgcgt ctggtggatg ttccgcgcct tcggccacca cgccgtgtca
      421 ctgcttgatg gcggcctccg ccactggctg cgccagaacc tcccgctcag ctccggcaag
      481 agccaacctg ctcccgccga gttccgcgct cagctcgacc ccgccttcat caagacctac
      541 gaggacatca aggagaacct ggaatcccgg cgcttccagg tggtggactc ccgagccact
      601 ggcaggttcc gcggcaccga gcccgagccc cgagacggca ttgaacctgg ccacatccca
      661 ggtaccgtga acatcccctt cacagacttc ctgagccagg aggggctgga gaagagccct
      721 gaggagatcc gccatctgtt ccaggagaag aaagtggacc tgtctaagcc actggtggcc
      781 acgtgtggct ctggcgtcac agcctgccac gtggcactag gggcctacct ctgcggcaag
      841 ccagacgtgc ccatctacga tggctcctgg gtggagtggt acatgcgcgc ccggcccgag
      901 gatgtcatct gacagtaaag gtggatacgg atccgaa
//
