LOCUS       sequence-57316-e         421 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 421)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NISCHA
FEATURES             Location/Qualifiers
     source          1..421
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggaagcgcg cgtcgtgggc tcggagcttg tggacactta tacggtttac
      121 atcatccagg tcactgatgg cagccatgag tggacagtaa agcaccgcta cagcgacttc
      181 catgacctgc atgaaaagct cgttgcagag agaaagattg ataaaaacct gcttccgccc
      241 aaaaagataa ttgggaaaaa ctcaagaagc ttggtggaga agagggagaa ggatctggag
      301 gtctacctcc agaagctcct ggctgccttc cctggcgtga cccccagagt actggcccac
      361 ttcttgcatt ttcacttcta tgagataaat ggctaacagt aaaggtggat acggatccga
      421 a
//
