LOCUS       sequence-57322-e         673 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 673)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 673)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIK3R2A
FEATURES             Location/Qualifiers
     source          1..673
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tgggcctcac actccccgac ttgcccgagc agttctcccc acctgatgtg
      121 gctccccctc ttctggtgaa gcttgtggag gccattgaaa ggacagggct ggacagcgaa
      181 tctcactacc gcccggagct gcccgcaccg cgtacagact ggtccctgag cgacgtggat
      241 cagtgggaca cggcagccct ggctgacggc attaagagct tcctgctggc actgcccgcg
      301 ccgctcgtga cccccgaggc ctcggccgag gcgcgccggg ccctgcggga ggccgcgggg
      361 cccgtggggc cggcgctgga gccaccgacg ctgccgctgc accgcgcgct cacgctgcgc
      421 ttcctgctcc agcacctggg ccgcgtggcc cgccgcgccc cggccctggg tcccgcggtc
      481 cgggccctgg gcgccacctt tgggccgctg ctgctgcgcg cgccgccgcc gccgtcctcg
      541 ccgccgccag ggggcgctcc cgacgggagt gagcccagcc ctgacttccc ggcgctgctg
      601 gtggagaagc tgcttcagga acacttggaa gagcaggagg ttgcgtgaca gtaaaggtgg
      661 atacggatcc gaa
//
