LOCUS       sequence-57326-e        1213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1213)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 1213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PIP4K2AA
FEATURES             Location/Qualifiers
     source          1..1213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             49..69
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 catatgcacc atcatcatca tcattcttct ggtgtagatc tgggtaccga gaacctgtac
       61 ttccaatcca tggacccgct gctcagcgtc ctcatgtggg gggtaaacca ctcgatcaat
      121 gaactgagcc atgttcaaat ccctgttatg ttgatgccag atgacttcaa agcctattca
      181 aaaataaagg tggacaatca cctttttaac aaagaaaaca tgccgagcca tttcaagttt
      241 aaggaatact gcccgatggt cttccgtaac ctgcgggaga ggtttggaat tgatgatcaa
      301 gatttccaga attccctgac caggagcgca cccctcccca acgactccca ggcccgcagt
      361 ggagctcgtt ttcacacttc ctacgacaaa agatacatca tcaagactat taccagtgaa
      421 gacgtggccg aaatgcacaa catcctgaag aaataccacc agtacatagt ggaatgtcat
      481 gggatcaccc ttcttcccca gttcttgggc atgtaccggc ttaatgttga tggagttgaa
      541 atatatgtga tagttacaag aaatgtattc agccaccgtt tgtctgtgta taggaaatac
      601 gacttaaagg gctctacagt ggctagagaa gctagtgaca aagaaaaggc caaagaactg
      661 ccaactctga aagataatga tttcattaat gagggccaaa agatttatat tgatgacaac
      721 aacaagaagg tcttcctgga aaaactaaaa aaggatgttg agtttctggc ccagctgaag
      781 ctcatggact acagtctgct ggtgggaatt catgatgtgg agagagccga acaggaggaa
      841 gtggagtgtg aggagaacga tggggaggag gagggcgaga gcgatggcac ccacccggtg
      901 ggaacccccc cagatagccc cgggaataca ctgaacagct caccacccct ggctcccggg
      961 gagttcgatc cgaacatcga cgtctatgga attaagtgcc atgaaaactc gcctaggaag
     1021 gaggtgtact tcatggcaat tattgacatc cttactcatt atgatgcaaa aaagaaagct
     1081 gcccatgctg caaaaactgt taaacatggc gctggcgcgg agatctccac cgtgaaccca
     1141 gaacagtatt caaagcgctt tttggacttt attggccaca tcttgtgaca gtaaaggtgg
     1201 atacggatcc gaa
//
