LOCUS       sequence-57340-e        9320 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GIGANTEA fused with Gal4 DNA binding domain (DBD), part of a 
            light-inducible transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9320)
  AUTHORS   Yazawa M, Sadaghiani AM, Hsueh B, Dolmetsch RE
  TITLE     Induction of protein-protein interactions in live cells using light.
  JOURNAL   Nat Biotechnol. 2009 Oct;27(10):941-5. doi: 10.1038/nbt.1569. Epub 
            2009 Oct 4.
  PUBMED    19801976
REFERENCE   2  (bases 1 to 9320)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.1569"; 
            journalName: "Nat Biotechnol"; date: "2009-10"; volume: "27"; issue:
            "10"; pages: "941-5"
COMMENT     Sequence Label: GI-Gal4 DBD
FEATURES             Location/Qualifiers
     source          1..9320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      944..953
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4487..4924
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="KLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKR
                     SPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKD
                     AVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     promoter        complement(4946..4964)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4947..4964)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4984..5001)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4990..5214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5260..5688
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5347..5366)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5557..5578
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5697..5717)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5702..6032
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5883..6018
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5945..5964
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6099..6893
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6153..6172)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6763..6782
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    7067..7188
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7104..7123)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7158..7177
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7237..7253)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7237..7253)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7250..7272)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7261..7277
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7285..7291,7292..7309,7310..7315))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7330..7351
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7468..7485)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7639..8227)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7719..7738)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8398..9189,9190..9258))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9021..9040
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(9259..9320,1..2))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat ccactagtaa cggccgccag tgtgctggaa ttcgccacca tggctagttc
      961 atcttcatct gagagatgga tcgatggtct tcagttctct tccttgttat ggcctccgcc
     1021 acgagatcct caacaacata aggatcaagt cgttgcttat gttgaatatt ttggtcaatt
     1081 tacatcagag caattcccag atgacattgc tgagttggtc cggcatcagt atccatcaac
     1141 cgagaagcga cttttggacg atgtgctggc gatgtttgtc cttcatcatc cggagcatgg
     1201 tcatgcagtc attcttccaa tcatttcatg tcttattgat ggctcgttgg tgtacagcaa
     1261 ggaagctcat ccgtttgcct ctttcatatc tttagtttgc ccaagtagtg agaatgacta
     1321 ttcggagcaa tgggctttgg catgtggaga aatccttcgc attttgactc attacaaccg
     1381 tcccatttat aaaactgagc agcaaaatgg agatacagag agaaattgtc tgagcaaagc
     1441 tacaactagt ggttctccga cttcagagcc taaggctgga tcaccaacac agcatgaaag
     1501 gaaaccttta aggcctttgt ctccatggat cagtgatata ctacttgctg ctcctcttgg
     1561 tataagaagt gactatttcc gatggtgtag tggtgtaatg ggtaaatatg ctgctggaga
     1621 gctcaagccg ccaaccattg cttctcgagg atctggtaaa catcctcaac tgatgccttc
     1681 aaccccaaga tgggctgttg ctaatggagc tggtgtcata ctgagtgttt gtgatgatga
     1741 agttgctcga tatgagactg ctacgctgac agcggtcgct gtccctgcac ttcttcttcc
     1801 tccgccaacg acatccttag atgagcatct agttgctggc cttccagctc ttgaaccata
     1861 tgcacgtttg tttcatagat actatgccat tgcaactcca agtgctacgc agagacttct
     1921 tcttggactc ttagaagcac caccgtcgtg ggctccagat gcacttgatg ctgctgtaca
     1981 gcttgtggaa ctccttcgag ctgctgaaga ttatgcatct ggtgtaaggc tacccaggaa
     2041 ctggatgcat ttgcacttct tgcgggctat aggaattgct atgtctatga gggcaggtgt
     2101 tgctgctgat gctgcagccg ctttgctttt ccgcatactc tcacagccgg cactgctttt
     2161 tcctccgcta agtcaagttg agggagtaga aattcagcac gcgcctattg gtggctacag
     2221 ttcaaattac agaaaacaga tagaagttcc tgcagcagaa gcaaccattg aagccactgc
     2281 ccaaggaatt gcctcaatgc tttgtgctca tggtcctgaa gttgagtgga gaatttgcac
     2341 tatatgggaa gctgcttatg gtttgatccc tttaaattct tcggcggttg atcttcccga
     2401 aatcatagtt gctaccccac tgcaacctcc tatcttgtca tggaatttat acattccact
     2461 cctcaaagta cttgaatatc ttccacgggg gagtccttcg gaagcatgct tgatgaaaat
     2521 atttgttgcc actgtggaaa caatactcag tagaactttt ccgcctgaat cttccaggga
     2581 actaaccaga aaagctagat cgagttttac cacaagatca gcgaccaaaa atcttgctat
     2641 gtctgagctt cgtgctatgg tccatgctct ctttttagaa tcatgcgctg gtgtggaatt
     2701 agcttcacgc ctactttttg ttgtgttgac tgtatgtgtt agccatgaag cacagtctag
     2761 tggtagcaag agaccgagaa gtgaatatgc tagtactact gaaaatattg aggcgaatca
     2821 acctgtatct aacaatcaaa ctgctaaccg taaaagtagg aatgtcaagg gacagggacc
     2881 tgtggcagca tttgattcat acgttcttgc tgctgtttgt gctcttgcct gtgaggttca
     2941 gctgtatcct atgatctctg gtggggggaa cttttccaat tctgccgtgg ctggaactat
     3001 tacaaagcct gtaaagataa atgggtcatc taaagagtat ggagctggga ttgactcggc
     3061 aattagtcat acgcgccgaa ttttggcaat cctagaggca ctcttttcat taaaaccatc
     3121 ttctgtgggg actccatgga gttacagttc tagtgagata gttgctgcgg ccatggttgc
     3181 agctcatatt tccgaactgt tcagacgttc aaaggccttg acgcatgcat tgtctgggtt
     3241 gatgagatgt aagtgggata aggaaattca taaaagagca tcatcattat ataacctcat
     3301 agatgttcac agcaaagttg ttgcctccat tgttgacaaa gctgaaccct tggaagccta
     3361 ccttaagaat acaccggttc agaaggattc tgtgacctgt ttaaactgga aacaagagaa
     3421 cacatgtgca agcaccacat gctttgatac agcggtgaca tccgcctcaa ggactgaaat
     3481 gaatccaaga ggaaaccata agtatgctag acattcagat gaaggctcag gaagaccctc
     3541 agagaagggt atcaaagatt tcctcttgga tgcttctgat ctagcgaatt tcctcacagc
     3601 tgatagactc gcagggttct attgtggtac acaaaagctt ttgaggtcag tgcttgcaga
     3661 gaaaccggag ctgtctttct ccgttgtttc actgttatgg cacaaactga ttgctgctcc
     3721 tgaaatccag cccaccgcag aaagcacctc tgcgcaacaa ggatggagac aggttgttga
     3781 tgcgctatgc aatgtcgtat ctgcaacgcc agcgaaagca gcagcagcag ttgtccttca
     3841 ggctgaaagg gagttgcagc cttggatcgc caaagatgat gaagaaggcc aaaaaatgtg
     3901 gaaaatcaac caacggatag tcaaagtgtt ggtggaactc atgcgcaatc atgacaggcc
     3961 tgagtcactg gtgattctcg caagtgcatc agatcttctt ctgcgggcaa ctgatggaat
     4021 gcttgttgat ggagaagctt gtacattacc tcaacttgag ctacttgaag ccacggcaag
     4081 agcaatacag ccggtgctag cttgggggcc atctggacta gcagtggtcg acggtttatc
     4141 caatctattg aagtgtcgtc taccagcaac aatacggtgc ctttcacacc caagtgcaca
     4201 cgtacgtgcc ttaagcacgt cagtactacg tgatatcatg aaccaaagct ccatacccat
     4261 caaagtaact ccaaaactgc caacaacaga gaagaacgga atgaatagtc cgtcctatcg
     4321 attcttcaac gccgcctcaa tagactggaa agccgatatc caaaactgtt taaactggga
     4381 agctcacagc ttgctctcca caactatgcc tactcagttt ctcgacactg cggctcggga
     4441 actcggctgt actatatcct tgtcccaagc ggccgcaagt gctggtaagc tactgtcttc
     4501 tatcgaacaa gcatgcgata tttgccgact taaaaagctc aagtgctcca aagaaaaacc
     4561 gaagtgcgcc aagtgtctga agaacaactg ggagtgtcgc tactctccca aaaccaaaag
     4621 gtctccgctg actagggcac atctgacaga agtggaatca aggctagaaa gactggaaca
     4681 gctatttcta ctgatttttc ctcgagaaga ccttgacatg attttgaaaa tggattcttt
     4741 acaggatata aaagcattgt taacaggatt atttgtacaa gataatgtga ataaagatgc
     4801 cgtcacagat agattggctt cagtggagac tgatatgcct ctaacattga gacagcatag
     4861 aataagtgcg acatcatcat cggaagagag tagtaacaaa ggtcaaagac agttgactgt
     4921 atcgccgtaa tctagagggc cctattctat agtgtcacct aaatgctaga gctcgctgat
     4981 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     5041 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5101 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5161 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5221 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     5281 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5341 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5401 aagctctaaa tcggggcatc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5461 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5521 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5581 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg gggatttcgg
     5641 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     5701 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca ggcaggcaga agtatgcaaa
     5761 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca
     5821 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc
     5881 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     5941 tttttattta tgcagaggcc gaggccgcct ctgcctctga gctattccag aagtagtgag
     6001 gaggcttttt tggaggccta ggcttttgca aaaagctccc gggagcttgt atatccattt
     6061 tcggatctga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa gatggattgc
     6121 acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg gcacaacaga
     6181 caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc ccggttcttt
     6241 ttgtcaagac cgacctgtcc ggtgccctga atgaactgca ggacgaggca gcgcggctat
     6301 cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc actgaagcgg
     6361 gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca tctcaccttg
     6421 ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat acgcttgatc
     6481 cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca cgtactcgga
     6541 tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg ctcgcgccag
     6601 ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc
     6661 atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct ggattcatcg
     6721 actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct acccgtgata
     6781 ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac ggtatcgccg
     6841 ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc tgagcgggac
     6901 tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag atttcgattc
     6961 caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat
     7021 gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc
     7081 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     7141 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat
     7201 accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7261 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7321 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7381 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7441 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7501 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7561 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7621 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7681 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7741 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7801 ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc
     7861 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     7921 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     7981 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8041 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8101 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8161 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8221 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8281 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8341 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8401 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8461 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8521 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8581 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8641 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8701 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8761 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8821 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     8881 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     8941 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9001 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9061 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9121 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9181 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9241 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9301 ttgtctcatg agcggataca
//
