LOCUS       sequence-57359-e         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Bergink S, Salomons FA, Hoogstraten D, Groothuis TA, de Waard H, Wu 
            J, Yuan L, Citterio E, Houtsmuller AB, Neefjes J, Hoeijmakers JH, 
            Vermeulen W, Dantuma NP
  TITLE     DNA damage triggers nucleotide excision repair-dependent 
            monoubiquitylation of histone H2A.
  JOURNAL   Genes Dev. 2006 May 15. 20(10):1343-52.
  PUBMED    16702407
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genes Dev. 
            2006 May 15. 20(10):1343-52."
COMMENT     Sequence Label: mRFP-Ub
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             29..703
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             29..703
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     primer_bind     complement(159..177)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     427..446
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     621..644
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 ttgaaccgtc agatccgcta gcaccaccat ggcctcctcc gaggacgtca tcaaggagtt
       61 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      121 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      181 cggccccctg cccttcgcct gggacatcct gtcccctcag ttccagtacg gctccaaggc
      241 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt
      301 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      361 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc
      421 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctccaccg agcggatgta
      481 ccccgaggac ggcgccctga agggcgagat caagatgagg ctgaagctga aggacggcgg
      541 ccactacgac gccgaggtca agaccaccta catggccaag aagcccgtgc agctgcccgg
      601 cgcctacaag accgacatca agctggacat cacctcccac aacgaggact acaccatcgt
      661 ggaacagtac gagcgcgccg agggccgcca ctccaccggc gccagat
//
