LOCUS       sequence-57367-e       11195 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11195)
  AUTHORS   Jang CW, Magnuson T
  TITLE     A Novel Selection Marker for Efficient DNA Cloning and 
            Recombineering in E. coli.
  JOURNAL   PLoS One. 2013;8(2):e57075. doi: 10.1371/journal.pone.0057075. Epub 
            2013 Feb 20.
  PUBMED    23437314
REFERENCE   2  (bases 1 to 11195)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013"; volume: "8(2):e57075. doi"; pages: " 
            10.1371/journal.pone.0057075. Epub 2013 Feb 20"
COMMENT     Sequence Label: pF2-DTA-Rosa26
FEATURES             Location/Qualifiers
     source          1..11195
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    31..1115
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    661..1307
                     /label=HA-L
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    1112..5381
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     promoter        5498..6676
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5729..6667
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6624..6644
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     primer_bind     6885..6904
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6979..7551
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          7628..7693
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(7761..7778)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7767..7991
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     promoter        complement(8038..8056)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8077..8093)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8077..8093)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8090..8112)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8101..8117
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8125..8131,8132..8149,8150..8155))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8170..8191
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8308..8325)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8479..9067)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8559..8578)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11116..11138
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11130..11147
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11131..11147
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11154..11173
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        11154..11172
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctcgaggccg gccttaagac gtcgctcccc gcggcaggcc ctccgagcgt ggtggagccg
       61 ttctgtgaga cagccgggta cgagtcgtga cgctggaagg ggcaagcggg tggtgggcag
      121 gaatgcggtc cgccctgcag caaccggagg gggagggaga agggagcgga aaagtctcca
      181 ccggacgcgg ccatggctcg gggggggggg ggcagcggag gancgcttcc ggccgacgtc
      241 tcgtcgctga ttggcttntt ttcctcccgc cgtgtgtgaa aacacaaatg gcgtgttttg
      301 gttggcgtaa ggcgcctgtc agttaacggc agccggagtg cgcagccgcc ggcagcctcg
      361 ctctgcccac tgggtggggc gggaggtagg tggggtgagg cgagctgnac gtgcgggcgc
      421 ggtcggcctc tggcggggcg ggggagggga gggagggtca gcgaaagtag ctcgcgcgcg
      481 agcggccgcc caccctcccc ttcctctggg ggagtcgttt tacccgccgc cggccgggcc
      541 tcgtcgtctg attggctctc ggggcccaga aaactggccc ttgccattgg ctcgtgttcg
      601 tgcaagttga gtccatccgc cggccagcgg gggcggcgag gaggcgctcc caggttccgg
      661 ccctcccctc ggccccgcgc cgcagagtct ggccgcgcgc ccctgcgcaa cgtggcagga
      721 agcgcgcgct gggggcgggg acgggcagta gggctgagcg gctgcggggc gggtgcaagc
      781 acgtttccga cttgagttgc ctcaagaggg gcgtgctgag ccagacctcc atcgcgcact
      841 ccggggagtg gagggaagga gcgagggctc agttgggctg ttttggaggc aggaagcact
      901 tgctctccca aagtcgctct gagttgttat cagtaaggga gctgcagtgg agtaggcggg
      961 gagaaggccg cacccttctc cggagggggg aggggagtgt tgcaatacct ttctgggagt
     1021 tctctgctgc ctcctggctt ctgaggaccg ccctgggcct gggagaatcc cttgccccct
     1081 cttcccctcg tgatctgcaa ctccagtctt tctagaagat gggcgggagt cttctgggca
     1141 ggcttaaagg ctaacctggt gtgtgggcgt tgtcctgcag gggaattgaa caggtgtaaa
     1201 attggaggga caagacttcc cacagatttt cggttttgtc gggaagtttt ttaatagggg
     1261 caaataggaa aatggaggat aggagtcatc tggggtttat gcagcaaaac tacaggtata
     1321 ttgcttgtat ccgcctcgga gatttccatg aggagataaa gacatgtcac ccgagtttat
     1381 actctcctgc ttagatccta ctacagtatg aaatacagtg tcgcgaggta gactatgtaa
     1441 gcagatttaa tcattttaaa gagcccagta cttcatatcc atttctcccg ctccttctgc
     1501 agccttatca aaaggtattt agaacactca ttttagcccc attttcattt attatactgg
     1561 cttatccaac ccctagacag agcattggca ttttcccttt cctgatctta gaagtctgat
     1621 gactcatgaa accagacaga ttagttacat acaccacaaa tcgaggctgt agctggggcc
     1681 tcaacactgc agttctttta taactcctta gtacactttt tgttgatcct ttgccttgat
     1741 ccttaatttt cagtgtctat cacctctccc gtcaggtggt gttccacatt tgggcctatt
     1801 ctcagtccag ggagttttac aacaatagat gtattgagaa tccaacctaa agcttaactt
     1861 tccactccca tgaatgcctc tctccttttt ctccattata actgagctat naccattaat
     1921 ggtttcaggt ggatgtctcc tcccccaata tacctgatgt atctacatat tgccaggctg
     1981 atattttaag acatnaaagg tatatttcat tattgagcca catggtattg attactgcta
     2041 ctaaaatttt gtcattgtac acatctgtaa aaggtggttc cttttggaat gcaaagttca
     2101 ggtgtttgtt gtctttcctg acctaaggtc ttgtgagctt gtattttttc tatttaagca
     2161 gtgctttctc ttggactggc ttgactcatg gcattctaca cgttattgct ggtctaaatg
     2221 tgattttgcc aagcttcttc aggacctata attttgcttg acttgtagcc aaacacaagt
     2281 aaaatgatta agcaacaaat gtatttgtga agcttggttt ttaggttgtt gtgttgtgtg
     2341 tgcttgtgct ctataataat actatccagg ggctggagag gtggctcgga gttcaagagc
     2401 acagactgct cttccagaag tcctgagttc aattcccagc aaccacatgg tggctcacaa
     2461 ccatctgtaa tgggatctga tgccctcttc tggtgtgtct gaagaccaca agtgtattca
     2521 cattaaataa ataatcctcc ttcttcttct tttttttttt ttaaagagaa tnctgtctcc
     2581 agtagaatta ctgaagtaat gaaatacttt gtgtttgttc caatatggna gccaataatc
     2641 aaatactctt nagcactgga aatgtaccaa ggaactattt tatttaagtg nactgtggac
     2701 agaggagcca taactgcaga cttgtgggat acagaagacc aatgcagact taatgtcttt
     2761 tctcttacac taagcaataa agaaataaaa attgaacttc tagtatccta tttgttaaac
     2821 tgctagcttt actaactttt gtgcttcatc tatacaaagc tgaaagctaa gtctgcagcc
     2881 attactaaac atgaaagcaa gtaatgataa ttttggattt caaaaatgta gggccagagt
     2941 ttagccagcc agtggtggtg cttgccttta tgccttaatc ccagcactct ggaggcagag
     3001 acaggcagat ctctgagttt gagcccagcc tggtctacac atcaagttct atctaggata
     3061 gccaggaata cacacagaaa ccctgttggg gaggggggct ctgagatttc ataaaattat
     3121 aattgaagca ttccctaatg agccactatg gatgtggcta aatccgtcta cctttctgat
     3181 gagatttggg tattattttt tctgtctctg ctgttggttg ggtcttttga cactgtgggc
     3241 tttcttaaag cctccttccc tgccatgtgg tctcttgttt gctactaact tcccatggct
     3301 taaatggcat ggctttttgc cttctaaggg cagctgctga gntttgcagc ctgatttcca
     3361 gggtggggtt gggaaatctt tcaaacacta aaattgtcct ttaatttttt tttaaaaaat
     3421 gggttatata ataaacctca taaaatagtt atgaggagtg aggtggacta atattaatga
     3481 gtccctcccc tataaaagag ctattaaggc tttttgtctt atactaactt tttttttaaa
     3541 tgtggtatct ttagaaccaa gggtcttaga gttttagtat acagaaactg ttgcatcgct
     3601 taatcagatt ttctagtttc aaatccagag aatccaaatt cttcacagcc aaagtcaaat
     3661 taagaatttc tgactttaat gttatttgct actgtgaata taaaatgata gcttttcctg
     3721 aggcagggtc tcactatgta tctctgcctg atctgcaaca agatatgtag actaaagttc
     3781 tgcctgcttt tgtctcctga atactaaggt taaaatgtag taatactttt ggaacttgca
     3841 ggtcagattc ttttataggg gacacactaa gggagcttgg gtgatagttg gtaaatgtgt
     3901 ttaagtgatg aaaacttgaa ttattatcac cgcaacctac tttttaaaaa aaaaagccag
     3961 gcctgttaga gcatgctaag ggatccctag gacttgctga gcacacaaga gtagtacttg
     4021 gcaggctcct ggtgagagca tatttcaaaa aacaaggcag acaaccaaga aactacagta
     4081 aggttacctg tctttaacca tctgcatata cacagggata ttaaaatatt ccaaataata
     4141 tttcattcaa gttttccccc atcaaattgg gacatggatt tctccggtga ataggcagag
     4201 ttggaaacta aacaaatgtt ggttttgtga tttgtgaaat tgttttcaag tgatagttaa
     4261 agcccatgag atacagaaca aagctgctat ttcgaggtct cttggttata ctcagaagca
     4321 cttctttggg tttccctgca ctatcctgat catgtgctag gcctncctta ggctgattgt
     4381 tgttcaaata acttaagttt cctgtcaggt gatgtcatat gatttcatat atcaaggcaa
     4441 aacatgttat atatgttaaa catttgnact taatgtgaaa gttaggtctt tgtgggtttt
     4501 gattttaatt tcaaaacctg agctaaataa gtcattttac atgtcttaca tttggtgaat
     4561 tgtatattgt ggtttgcagg caagactctc tgacctagta accctcctat agagcacttt
     4621 gctgggtcac aagtctagga gtcaagcatt tcaccttgaa gttgagacgt tttgttagtg
     4681 tatactagtt atatgttgga ggacatgttt atccagaaga tattcaggac tatttttgac
     4741 tgggctaagg aattgattct gattagcact gttagtgagc attgagtggc ctttaggctt
     4801 gaattggagt cacttgtata tctcaaataa tgctggcctt ttttnaaaag cccttgttct
     4861 ttatcaccct gttttctaca taatttttgt tcaaagaaat acttgtttgg atctcctttt
     4921 gacaacaata gcatgttttc aagccatatt ttttttcctt tttttttttt tttttggttt
     4981 ttcgagacag ggtttctctg tatagccctg gctgtcctgg aactcacttt gtagaccagg
     5041 ctggcctcga actcagaaat ccgcctgcct ctgcctcctg agtgccggga ttaaaggcgt
     5101 gcaccaccac gcctggctaa gttggatatt ttgtatataa ctataaccaa tactaactcc
     5161 actgggtgga tttttaattc agtcagtagt cttaagtggt ctttattggc ccttattaaa
     5221 atctactgtt cactctaaca gaggctgttg gactagtggn actaagcaac ttcctacgga
     5281 tatactagca gataagggtc agggatagaa actagtctag cgttttgtat acctaccagc
     5341 ttatactacc ttgttctgat agaaatattt aggacatcta gcttatcgat ccgtcgacaa
     5401 gcttgaattc gcggccgctt aattaaggcg cgccgtttag cttgatttat aaaggtagca
     5461 gatctggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
     5521 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
     5581 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
     5641 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
     5701 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gckggcctgg
     5761 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
     5821 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
     5881 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
     5941 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
     6001 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
     6061 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
     6121 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
     6181 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
     6241 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
     6301 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
     6361 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     6421 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     6481 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     6541 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     6601 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     6661 atttcaggtg tcgtgaggaa ttagcttggt aggatccact agtctcgtgc agatggacag
     6721 caccgctgag caatggaagc gggtaggcct ttggggcagc ggccaatagc agctttgctc
     6781 cttcgctttc tgggctcaga ggctgggaag gggtgggtcc gggggcgggc tcaggggcgg
     6841 gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc ccggcattct gcacgcttca
     6901 aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct ccgggccttt cgacctgcag
     6961 gtcctcgcca tggatcctga tgatgttgtt gattcttcta aatcttttgt gatggaaaac
     7021 ttttcttcgt accacgggac taaacctggt tatgtagatt ccattcaaaa aggtatacaa
     7081 aagccaaaat ctggtacaca aggaaattat gacgatgatt ggaaagggtt ttatagtacc
     7141 gacaataaat acgacgctgc gggatactct gtagataatg aaaacccgct ctctggaaaa
     7201 gctggaggcg tggtcaaagt gacgtatcca ggactgacga aggttctcgc actaaaagtg
     7261 gataatgccg aaactattaa gaaagagtta ggtttaagtc tcactgaacc gttgatggag
     7321 caagtcggaa cggaagagtt tatcaaaagg ttcggtgatg gtgcttcgcg tgtagtgctc
     7381 agccttccct tcgctgaggg gagttctagc gttgaatata ttaataactg ggaacaggcg
     7441 aaagcgttaa gcgtagaact tgagattaat tttgaaaccc gtggaaaacg tggccaagat
     7501 gcgatgtatg agtatatggc tcaagcctgt gcaggaaatc gtgtcaggcg atctctttgt
     7561 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     7621 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     7681 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcaga
     7741 tcctagagct cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     7801 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     7861 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     7921 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     7981 gggctctatg gcttctgagg cggaaagaac cagctggggc tcgaccagct tttgttccct
     8041 ttagtgaggg ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     8101 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     8161 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     8221 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     8281 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8341 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8401 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8461 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8521 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8581 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8641 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8701 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8761 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8821 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8881 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8941 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9001 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9061 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9121 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     9181 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     9241 ccaatgctta atcagtgagg ccgcccatct ttaccaacag aacgattatt tcagttcgag
     9301 ttcgttcatt gcagcaatgc tgaaaccgcc gtcaacgtgg accacttcac cggagatacc
     9361 ggcagagaga tcggagcaca ggaatgccgc agagttaccc acatcttcaa tagtaacggt
     9421 acggcgaatc ggggtaacgg cttcgcaatg agccagcatt ttgcggaagt ctttgatacc
     9481 ggaggccgcc agagtacgga tcggaccagc agagatggcg ttaacacgca caccttccgg
     9541 acccatcgcg ttcgccatat agcgcacgtt cgcttccaga gacgcttttg ccagacccat
     9601 aacgttgtag ttcgggatag cgcgctcagc gccaaggtag gaaagggtca gcagggcaga
     9661 acccggattc agcatggagc ggcaagcttt tgccattgca acgaagctgt aggagctgat
     9721 gtcgtgggca attttgaagc cttcacgggt aacggcgtta acatagtcac catccagctg
     9781 atcgccaggt gcaaaaacaa tagagtgtac gaaaccgtca aatttcggcc aaactttccc
     9841 cagttcagcg aacatggtgt cgatgctggc atcttctgca acatcgcact gcagaacgat
     9901 gtcagaaccc aattgagcgg caaattcttc tacgcggcct ttcagtttgt cgttctggta
     9961 ggtgaatgcc agttcagctc cttcgcggtg catcgcctga gcgataccgt aggcgatgga
    10021 tagtttgctg gcaacaccgg ttaccagaat gcgcttaccg gaaagaaaac ccatagcttt
    10081 aatccttatt gttgatgctt gttgtgcctg aaaatcaggc gattcgtcgt tttagtaaac
    10141 agtacgaaca gataaacggt tattataatc aacctggctg tgagtagcta tagttgccag
    10201 gtccgaccgg agcaggctgc ggcagggggg gcgcttttcc cctcacccta accctctccc
    10261 cagaggggcg aggggaccgt attgtgcaaa tattgttacc ccagcaacaa acaggctcat
    10321 acagccccta accctttcat ggcgatggct gggacggttc agaccttgcc gaatattctc
    10381 cagcaccgtc tccatgtttt caccacaaca gctattcggc tcggtctcag ggttattgtc
    10441 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
    10501 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
    10561 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
    10621 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
    10681 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
    10741 agggtgatgg ttcacgtagt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc
    10801 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc
    10861 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg
    10921 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac
    10981 agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg
    11041 cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg
    11101 taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg
    11161 actcactata gggcgaattg ggtaccgggc ccccc
//
