LOCUS       sequence-57376-e         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid is used to reconstitute the complete Type II CRISPR 
            system from S. pyogenes and contains the host factor RNaseIII and 
            tracrRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, 
            Jiang W, Marraffini LA, Zhang F
  TITLE     Multiplex Genome Engineering Using CRISPR/Cas Systems.
  JOURNAL   Science. 2013 Jan 3.
  PUBMED    23287718
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 Jan 3."
COMMENT     Sequence Label: pX097-U6-SpTracrRNA-EF1a-hSpRNaseIII-mCherry
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     125..144
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        203..281
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
     misc_feature    337..454
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        598..809
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
ORIGIN
        1 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
       61 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
      121 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
      181 cttgtggaaa ggacgaaaca ccggaaccat tcaaaacagc atagcaagtt aaaataaggc
      241 tagtccgtta tcaacttgaa aaagtggcac cgagtcggtg ctttttttct agcgcgtgcg
      301 ccaattctgc agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
      361 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
      421 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
      481 tccagtttgg ttaattaatg caaagatgga taaagtttta aacagagagg aatctttgca
      541 gctaatggac cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg
      601 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgatccg
      661 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
      721 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
      781 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
      841 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
      901 tgattcttga tcccgagctt cgggttggaa gtg
//
